Protein Info for Atu4173 in Agrobacterium fabrum C58

Annotation: phosphoenolpyruvate-protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 755 PF13185: GAF_2" amino acids 24 to 160 (137 residues), 59.2 bits, see alignment E=1.3e-19 PF01590: GAF" amino acids 25 to 157 (133 residues), 72.6 bits, see alignment E=1.2e-23 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 184 to 731 (548 residues), 416.7 bits, see alignment E=6.7e-129 PF05524: PEP-utilisers_N" amino acids 185 to 303 (119 residues), 77.1 bits, see alignment E=3.4e-25 PF00391: PEP-utilizers" amino acids 331 to 403 (73 residues), 53.6 bits, see alignment E=3.6e-18 PF02896: PEP-utilizers_C" amino acids 430 to 716 (287 residues), 303.6 bits, see alignment E=3.5e-94

Best Hits

KEGG orthology group: K08484, phosphotransferase system, enzyme I, PtsP [EC: 2.7.3.9] (inferred from 100% identity to atu:Atu4173)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase, nitrogen regulation associated"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CG69 at UniProt or InterPro

Protein Sequence (755 amino acids)

>Atu4173 phosphoenolpyruvate-protein phosphotransferase (Agrobacterium fabrum C58)
MRDLSAGPRVLLKRLREMMAEHLEPQERLDQIVRQIASNMVAEVCSVYVLRSDSVLELYA
TEGLNKDAVHLAQLKMGQGLVGTIAASAQPLNLSDAQAHPAFRYLPETGEEIYHSFLGVP
ILRSGRTLGVLVVQNKASRTYREDEVEALETTAMLIAEIVASGELKKITRPGVELDLTRA
VSIDGDAYGEGIGLGHVVLHDPRIVVTNLLNEDADTEIRRLADAIGSLRLSIDDMLQRRD
VPTEGEHREVLETYRMFAHDQGWVRRMEEAIRNGLTAEAAVEKVQSDTKARMMRLTDPYL
RERMHDFDDLANRLLRQLIGFGGHNPAQDFPLDAIVLARAMGAAELLDYPREKLRGLVLE
DGAVTSHVVIVARAMGIPIIGQATGIVALAENNDPIIIDGDDGQVHLRPMSDLQRAYEEK
VRLRAKRQEQFRALRNVEPITKDGQKVNLKMNAGLLVDLPQLEESGADGIGLFRTELQFM
IASNMPKGEEQEAFYRSVLRQAKGKSVTFRTLDIGGDKVVSYMRGQEEENPALGWRAIRL
SLDRPGLMRTQMRALLRAASGAELRMMLPMVTEVSEIRAARDLLQKEVQHLSKFSHALPK
KLQFGAMLEVPSLMWQLDELMQEVDFVSVGSNDLFQFSMAVDRGNARVSDRFDNLGKPFL
RILRDIVRAGEKHHTAVTLCGEMASKPLSAMALIGLGFRSVSMSPTAIGPVKAMLLGLDA
ANLAEELNAALDDHNSLESAREVLLRFAATHSIPI