Protein Info for Atu4166 in Agrobacterium fabrum C58

Annotation: UDP-glucose 4-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01179: UDP-glucose 4-epimerase GalE
  • amino acids 5 to 326 (322 residues), 429.6 bits, E=3.3e-133
• PF04321: RmlD_sub_bind
  • amino acids 5 to 158 (154 residues), 56.7 bits, E=6.1e-19
• PF01370: Epimerase
  • amino acids 6 to 253 (248 residues), 185.2 bits, E=4.3e-58
• PF02719: Polysacc_synt_2
  • amino acids 6 to 175 (170 residues), 51.7 bits, E=2.2e-17
• PF01073: 3Beta_HSD
  • amino acids 7 to 154 (148 residues), 44.4 bits, E=3.4e-15
• PF16363: GDP_Man_Dehyd
  • amino acids 7 to 315 (309 residues), 194.7 bits, E=9.1e-61
• PF07993: NAD_binding_4
  • amino acids 70 to 182 (113 residues), 26.8 bits, E=8.5e-10

Best Hits

Swiss-Prot: 79% identical to EXOB_RHIME: UDP-glucose 4-epimerase (exoB) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 100% identity to atu:Atu4166)

MetaCyc: 56% identical to UDP-xylose 4-epimerase subunit (Sinorhizobium meliloti 1021)
UDP-arabinose 4-epimerase. [EC: 5.1.3.5]

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (5/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (2/2 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-β-L-arabinose biosynthesis I (from UDP-α-D-xylose) (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• stachyose degradation (4/7 steps found)
• D-galactose detoxification (1/3 steps found)
• UDP-sugars interconversion (4/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (2/18 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2 or 5.1.3.5

Sequence Analysis Tools

See Q7CU82 at UniProt or InterPro

Protein Sequence (328 amino acids)

>Atu4166 UDP-glucose 4-epimerase (Agrobacterium fabrum C58)
MMKKKVLVVGGAGYIGSHTCLLLSERGYEPVVFDNLSNGHEEFVRWGPFEQGDIRDRARL
DEVFAKHRPEAVLHFAALIEVGESVKQPVAFYDNNVIGSLNLLSAAIDAGVTSFVFSSTC
ATYGLPEQVPIDETHRQAPINPYGRTKWVVEQALKDYSTYEGLRSVMLRYFNAAGADFEG
RIGEWHKPETHAIPLAIEAALGRRQGFKVFGTDYDTRDGTCVRDYIHILDLADAHVRAVD
YLLDGGETVELNLGTGTGTTVKELLAAISEVSGRPFPVEYTGRRDGDSTTLVANNDKARE
VLGWEPRYSLSDIIKSAWAWHSSRNAGD