Protein Info for Atu4153 in Agrobacterium fabrum C58

Annotation: aldehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 509 transmembrane" amino acids 149 to 169 (21 residues), see Phobius details PF00171: Aldedh" amino acids 31 to 487 (457 residues), 428.2 bits, see alignment E=1.7e-132

Best Hits

Swiss-Prot: 53% identical to AL7A1_PEA: Aldehyde dehydrogenase family 7 member A1 from Pisum sativum

KEGG orthology group: K00128, aldehyde dehydrogenase (NAD+) [EC: 1.2.1.3] (inferred from 100% identity to atu:Atu4153)

MetaCyc: 68% identical to L-aminoadipate-semialdehyde dehydrogenase (Pseudomonas putida)
L-aminoadipate-semialdehyde dehydrogenase. [EC: 1.2.1.31]

Predicted SEED Role

"Aldehyde dehydrogenase B (EC 1.2.1.22)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Methylglyoxal Metabolism (EC 1.2.1.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.22, 1.2.1.3

Use Curated BLAST to search for 1.2.1.22 or 1.2.1.3 or 1.2.1.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CU70 at UniProt or InterPro

Protein Sequence (509 amino acids)

>Atu4153 aldehyde dehydrogenase (Agrobacterium fabrum C58)
MDAMTKLDVKQEAAALLDKMGVARDLYTGGDMASFSPVTGEQIASLRTVSVEGVAAVVDK
ADAAFRTWRNVPAPRRGELIRLLGEELRAFKTDLGRLVSLEAGKIPSEGLGEVQEMIDIC
DFAVGLSRQLYGLTIATERPGHRMMETWHPLGVVGVISAFNFPVAVWSWNAALALVCGNS
VVWKPSEKTPLTALAVQGIFERAAARFGDAPEGLSQLLIGDRAVGEAMVDYPKVPLVSAT
GSTRMGRDVGPRLAKRFARAILELGGNNAGIVCPSADLDMALRAIAFGAMGTAGQRCTTL
RRLFVHDSVYEELVPRLKKAYASVSVGNPLESSALVGPLVDKAAFDGMQKAIEAAKAAGG
VVHGGDRVDTGAADAYYVKPALVEMPKQVGPVLEETFAPILYVMKYSDLDQAIDAHNAVA
AGLSSSIFTRDIQESERFLSSEGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDS
WKAYMRRATNTINYSKALPLAQGVSFDIE