Protein Info for Atu4130 in Agrobacterium fabrum C58

Annotation: acetoacetyl-CoA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 651 TIGR01217: acetoacetate-CoA ligase" amino acids 8 to 651 (644 residues), 798 bits, see alignment E=2.8e-244 PF00501: AMP-binding" amino acids 105 to 474 (370 residues), 132.7 bits, see alignment E=1.5e-42 PF13193: AMP-binding_C" amino acids 543 to 613 (71 residues), 35.4 bits, see alignment E=1.7e-12

Best Hits

Swiss-Prot: 55% identical to AACS_RHIME: Acetoacetyl-coenzyme A synthetase (acsA2) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01907, acetoacetyl-CoA synthetase [EC: 6.2.1.16] (inferred from 100% identity to atu:Atu4130)

Predicted SEED Role

"Acetoacetyl-CoA synthetase (EC 6.2.1.16)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Polyhydroxybutyrate metabolism (EC 6.2.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CG45 at UniProt or InterPro

Protein Sequence (651 amino acids)

>Atu4130 acetoacetyl-CoA synthetase (Agrobacterium fabrum C58)
MMEAMPSDILWVPPRERLEHSALFAFAAKTAPLHGLAADDYAGLLRWSIDAPDEFYDALW
DELAIIGTRGEAAFKAGATIRDAEFYPGARLNYAENLLRDADERLAIIAYRDDGTRREIS
RRKLYDEVSRMVQALVHAGVKEGDRVGAIVTHDIEAIVSYLAVSAIGAIWSSCSPDFGPA
GASDRLSQIDPKILIAVPEYGYAGKRIDVGPTIRAVAESARPEKIVLIGDSVAESLADLG
CLTLADFVAPYAAGEIAFNRLPVKAPLAILYSSGTTGKPKCITHSGGGLLLQHMKEQKLQ
CDIRQGERFFYFTTCGWMMWNWQVSGLALGATLVTYDGNPGYPNTARLIDLIDAEGIATF
GTSAKFIDACLKAGLQPRESHDLSSLRTILSTGSPLIPQSFDYIYRDWRADLHLASISGG
TDICACFLGGNPLQSVHRGELQGAMLGMDIDTLDDEGRSVRGVAAELVCRNAHLSMPVKF
WGDEDGSRYQAAYFDRFPGIWAHGDFAELRPSGGYVIHGRSDTTLNPGGVRIGTAEIYRQ
VETVPDVEEAIAVGQDIDGDQRVILFIRMKEGAVLTEELEKTIRSRIRLGATPRHVPARI
IAVSAIPRTRSGKISEIAVRETIHGRPVKNTDALANPESLELFRNLPQLKD