Protein Info for Atu4077 in Agrobacterium fabrum C58

Annotation: glycogen branching protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 735 PF22019: GlgB_N" amino acids 18 to 108 (91 residues), 55.8 bits, see alignment E=8e-19 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 118 to 731 (614 residues), 887.3 bits, see alignment E=2.7e-271 PF02922: CBM_48" amino acids 134 to 217 (84 residues), 78.1 bits, see alignment E=1e-25 PF00128: Alpha-amylase" amino acids 286 to 369 (84 residues), 33.2 bits, see alignment E=7.3e-12 PF02806: Alpha-amylase_C" amino acids 639 to 733 (95 residues), 80.5 bits, see alignment E=1.8e-26

Best Hits

Swiss-Prot: 100% identical to GLGB_AGRFC: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 100% identity to atu:Atu4077)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.18

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8U8L4 at UniProt or InterPro

Protein Sequence (735 amino acids)

>Atu4077 glycogen branching protein (Agrobacterium fabrum C58)
MKKPLNSAEEKKTGDITKAEIEAIKSGLHSNPFALLGVHETPEGFSARCFIPGAEEVSVL
TLDGNFVGELKQIDPDGFFEGRIDLSKRQPVRYRACRDDAEWAVTDPYSFGPVLGPMDDY
FVREGSHLRLFDRMGAHPLKLEGVEGFHFAVWAPNARRVSVVGDFNNWDGRRHVMRFRKD
TGIWEIFAPDVYAGCAYKFEILGANGELLPLKADPYARRGELRPKNASVTAPELTQKWED
QAHREHWAQVDQRRQPISIYEVHAGSWQRREDGTFLSWDELAAQLIPYCTDMGFTHIEFL
PITEHPYDPSWGYQTTGLYAPTARFGDPEGFARFVNGAHKVGIGVLLDWVPAHFPTDEHG
LRWFDGTALYEHADPRQGFHPDWNTAIYNFGRIEVMSYLINNALYWAEKFHLDGLRVDAV
ASMLYLDYSRKEGEWIPNEYGGRENLESVRFLQKMNSLVYGTHPGVMTIAEESTSWPKVS
QPVHEGGLGFGFKWNMGFMHDTLSYFSREPVHRKFHHQELTFGLLYAFTENFVLPLSHDE
VVHGKGSLIAKMSGDDWQKFANLRSYYGFMWGYPGKKLLFMGQEFAQWSEWSEKGSLDWN
LRQYPMHEGMRRLVRDLNLTYRSKAALHARDCEPDGFRWLVVDDHENSVFAWLRTAPGEK
PVAVICNLTPVYRENYYVPLPVAGRWREILNTDAEIYGGSGKGNGGRVQAVDAGGEIGAM
LVLPPLATIMLEPEN