Protein Info for Atu4069 in Agrobacterium fabrum C58

Annotation: sarcosine oxidase alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 986 TIGR01372: sarcosine oxidase, alpha subunit family" amino acids 4 to 985 (982 residues), 1506.4 bits, see alignment E=0 PF13510: Fer2_4" amino acids 18 to 102 (85 residues), 81.3 bits, see alignment E=1.9e-26 PF07992: Pyr_redox_2" amino acids 172 to 435 (264 residues), 47.9 bits, see alignment E=5.7e-16 PF00890: FAD_binding_2" amino acids 172 to 214 (43 residues), 28.3 bits, see alignment (E = 4.7e-10) PF12831: FAD_oxidored" amino acids 172 to 232 (61 residues), 41.2 bits, see alignment 6.2e-14 PF01266: DAO" amino acids 172 to 421 (250 residues), 32.5 bits, see alignment E=3.1e-11 PF13450: NAD_binding_8" amino acids 175 to 209 (35 residues), 22.3 bits, see alignment (E = 5.9e-08) PF17806: SO_alpha_A3" amino acids 505 to 585 (81 residues), 101.7 bits, see alignment E=1e-32 PF01571: GCV_T" amino acids 603 to 868 (266 residues), 228.2 bits, see alignment E=4.8e-71 PF08669: GCV_T_C" amino acids 893 to 978 (86 residues), 62.5 bits, see alignment E=1.3e-20

Best Hits

KEGG orthology group: K00302, sarcosine oxidase, subunit alpha [EC: 1.5.3.1] (inferred from 100% identity to atu:Atu4069)

Predicted SEED Role

"Sarcosine oxidase alpha subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CG08 at UniProt or InterPro

Protein Sequence (986 amino acids)

>Atu4069 sarcosine oxidase alpha subunit (Agrobacterium fabrum C58)
MSGDYRIKGAGRLTPARTARFTFDGKIYPALEGDTVASALLANGVHLIGRSFKYHRPRGF
LSAGPEEPNALIDVSRDSLRKQPNVRATVQEVFDGAIIASQNRFPSLSFDLSAVNDLLSP
MFAAGFYYKTFMWPKAAWHKIYEPLIRRAAGLGKAPSEPDADHYSGRYAHCDVLVAGAGV
AGLTAALSAAKTGASVILVDENAEIGGALRFDTGAVIDGLPGYEWAQKVLAELQSMSNVR
VLTRTTAFGYYNHNFVALAERVTDHLATPARHQPRERLWQVRAKKVVLAAGAIERHMVFA
NNDRPGIMLAAAARTYLNQYGVSVGHNVGVYTAHDSAYETAFDLKKAGVKIAAIVDCREN
PDQRLLDEARALGIEVLAGHSVYNTAGRLRVSSMTVGRNGGSNKRKIAIDALVVSAGWTP
SVHLFSQSRGKLKFDAANQRFLPDIHVQNCVSIGACNGTDDLVAIIAEAAAAGGGSVAFS
GENARAWTGGMIGAAEGAGEGTGVKAFIDFQHDVCAKDIRLAVREGMHSVEHIKRFTTNG
MASDQGKMSNMHGLAIASEALGRDLPKVGLTTFRQPYTPVTFGTLINHSRGALFDPTRKT
PMHEEELAAGAVFEDVGNWKRAWFFPRAGEDMHEAINRECKTVRTSVGVFDASTLGKIEV
VGPDAAKFLNLIYTNAWDTLKPGRCRYGIMTREDGFVYDDGVVGRLAEDRFHVTTTTGGA
PRVLQHMEDYLQTEFPDLNVWLTSATEQWAVIAVQGPKAREVIAPFVEGIDLSPEAFPHM
AVAEGKFCGVPTRLFRVSFTGELGFEINVPADYGAAVWSAIRDRTEAVGGCLYGTETMHI
LRAEKGYIIVGQDTDGTVTPDDAGLAWAVSKKKTDFVGIRGLKRIDLTRTGRKQLVGLKT
KDRLTVPDEGGQIVTDPNQPKPMTMLGHVTSAYWSENLGHSIAFALVADGRARMGETLYI
PLADKTIAVEVTDMVFLDKEGTRLNG