Protein Info for Atu3997 in Agrobacterium fabrum C58

Annotation: biotin synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 339 TIGR00433: biotin synthase" amino acids 30 to 326 (297 residues), 404.8 bits, see alignment E=1.1e-125 PF04055: Radical_SAM" amino acids 63 to 221 (159 residues), 88.4 bits, see alignment E=6.3e-29 PF06968: BATS" amino acids 235 to 326 (92 residues), 97.5 bits, see alignment E=4.1e-32

Best Hits

Swiss-Prot: 100% identical to BIOB_AGRFC: Biotin synthase (bioB) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01012, biotin synthetase [EC: 2.8.1.6] (inferred from 100% identity to atu:Atu3997)

MetaCyc: 64% identical to biotin synthase (Escherichia coli K-12 substr. MG1655)
Biotin synthase. [EC: 2.8.1.6]

Predicted SEED Role

"Biotin synthase (EC 2.8.1.6)" in subsystem Biotin biosynthesis (EC 2.8.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CFX5 at UniProt or InterPro

Protein Sequence (339 amino acids)

>Atu3997 biotin synthetase (Agrobacterium fabrum C58)
MDQLATQIDGKPASIPAVETSSSLEEAKIIYNLPFNDLLFRAQQVHRCHFDANAIQMSRL
LSIKTGGCPEDCSYCSQSARNPTGLKASKLMEVERVLAEARKAKEGGATRYCMGAAWRNP
KERDMEAVVAMVEGVKALDMETCMTLGMLTPEQSERLADAGLDYYNHNVDTSERFYSEII
TTRTFEDRLETLANVRDAGIKVCAGGILGMGETVEDRISMLVTLANLPVPPESVPINMLI
PIPGSKLANADPVDPIDFVRTIALARILMPRSHVRLSAGRTEMSDETQALCFLAGANSIF
IGETLLTADNPGEDHDTALFRRLGLKPMELQSSEAGGCR