Protein Info for Atu3933 in Agrobacterium fabrum C58

Annotation: histidine ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 514 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR01225: histidine ammonia-lyase" amino acids 6 to 508 (503 residues), 729.1 bits, see alignment E=1.1e-223 PF00221: Lyase_aromatic" amino acids 14 to 472 (459 residues), 611.5 bits, see alignment E=5e-188

Best Hits

Swiss-Prot: 100% identical to HUTH_AGRFC: Histidine ammonia-lyase (hutH) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 100% identity to atu:Atu3933)

MetaCyc: 54% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]

Predicted SEED Role

"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.3

Use Curated BLAST to search for 4.3.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8U8Z7 at UniProt or InterPro

Protein Sequence (514 amino acids)

>Atu3933 histidine ammonia-lyase (Agrobacterium fabrum C58)
MTMKTITLHPGAVTLADLAAIYWENGTAKLDRSFDAGIEKAAARIAEIAAGNAPVYGINT
GFGKLASIKIDAADVATLQRNLILSHCCGVGVPLPENVVRLIMALKLVSLGRGASGVRLE
LVRLIEAMLEKGVIPVIPEKGSVGASGDLAPLAHMAAVMMGEGEAFYEGAPLPAGEALAK
AGLTPVVLAAKEGLALINGTQTSTALALAGLFRAHRAAQAALITGALSTDAAMGSSAPFH
PDIHSLRGHKGQIDAGAALRNLLEGSEIRVSHIEGDERVQDPYCIRCQPQVDGACLDLLR
QVARTLEIEANAVTDNPLVLSDNSVVSGGNFHAEPVAFAADQTALAICEIGAIAQRRVAL
LVDPALSYGLPAFLSKKPGLNSGLMIAEVTSAALMSENKQMAHPASVDSTPTSANQEDHV
SMACHGARRLLPMTDNLFAILGIEALSAVQGVELRGPLKTSPELQKVIAVLRVVVPSLEE
DRYMAPDLKAAAELIASGALVSTISGGILPKLEA