Protein Info for Atu3905 in Agrobacterium fabrum C58

Annotation: nitrate ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 signal peptide" amino acids 1 to 38 (38 residues), see Phobius details PF13379: NMT1_2" amino acids 41 to 300 (260 residues), 362.2 bits, see alignment E=9.7e-113

Best Hits

Swiss-Prot: 60% identical to NRTA_SYNE7: Nitrate/nitrite binding protein NrtA (nrtA) from Synechococcus elongatus (strain PCC 7942)

KEGG orthology group: K02051, sulfonate/nitrate/taurine transport system substrate-binding protein (inferred from 100% identity to atu:Atu3905)

Predicted SEED Role

"Nitrate ABC transporter, nitrate-binding protein" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CTL1 at UniProt or InterPro

Protein Sequence (430 amino acids)

>Atu3905 nitrate ABC transporter substrate-binding protein (Agrobacterium fabrum C58)
MKKIFSGTVSRRTILKTTATAALVTAVRTAFPSGAFAATAEPEVKGAKLGFIALTDAAPL
IIAAEKGLFAKHGMPEVEVLKQASWGATRDNLVLGGASNGIDGAHILTPMPYLMHTGKVT
QNNVPVPMTILARLNLDSQGISVAKEYAETGVQLDASKLKAAFEKKKADGKEIKAAMTFP
GGTHDLWIRYWLAAGGIDPDKDVSTIVVPPPQMVANMKVGNMDVFCVGEPWNEQLVNQGI
GFTACTTGELWKGHPEKALGMRADWVEKNPNATKALLMAVMEAQQWCDEMANKEEMSTIL
GKRQWFNVPPKDVLGRLKGNINYGNGRVLENTGLQMKFWQDHASYPFHSHDSWFITENIR
WGKFAPDTDVKALVAKVNREDIWRAAAKDLGVADLPASTSRGKETFFDGKVFDPENPSAY
LESLSIKAAS