Protein Info for Atu3902 in Agrobacterium fabrum C58

Annotation: nitrite reductase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 815 PF07992: Pyr_redox_2" amino acids 4 to 281 (278 residues), 187 bits, see alignment E=2.4e-58 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 5 to 793 (789 residues), 894.2 bits, see alignment E=4.6e-273 PF13738: Pyr_redox_3" amino acids 64 to 256 (193 residues), 44.4 bits, see alignment E=5.9e-15 PF00070: Pyr_redox" amino acids 146 to 226 (81 residues), 63.9 bits, see alignment E=7.2e-21 PF18267: Rubredoxin_C" amino acids 316 to 382 (67 residues), 66.3 bits, see alignment E=8.7e-22 PF04324: Fer2_BFD" amino acids 419 to 466 (48 residues), 44.5 bits, see alignment 7.2e-15 amino acids 482 to 531 (50 residues), 45.3 bits, see alignment 4e-15 PF03460: NIR_SIR_ferr" amino acids 557 to 620 (64 residues), 55.1 bits, see alignment 2.4e-18 PF01077: NIR_SIR" amino acids 630 to 770 (141 residues), 115.3 bits, see alignment E=8.3e-37

Best Hits

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 100% identity to atu:Atu3902)

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CTK8 at UniProt or InterPro

Protein Sequence (815 amino acids)

>Atu3902 nitrite reductase large subunit (Agrobacterium fabrum C58)
MTQKLVIIGNGMAPGRMLENLFETAPGLYDVTIFNAEPRVNYDRIMLSPVLSGEKSYEDI
VIHNDEWYAANNVTLHKGAKVTRIDRANKIVTSENGITASYDRLVIATGSLPFIIPVPGH
QLPGVLAYRDLDDVTKMLEIAKGKGRAIVIGAGLLGLEAAYGLKRQGMDVTVIHLMPTIM
ERQLDPAAAYLLEKALNERGIDIITKANTKCILGEEKVEGIELEDGRIIKGDMVVMAVGI
RPASGLAKEAGIAVNRGIVVDDGMMTSDASIYALGECAEHRGMCYGLVAPLYESARVLAD
RLCGGSAEYHGSVTNTKLKVTGINLFSAGDFAEGDDREEIVLRDATAGVYKRLILKENRI
IGAVLYGETADGSWFFDLMKKSTDISAMRETLIFGQAYQGGSPLDPMAAVAALPDDAEIC
GCNGVCKGKITSAITSKGLTSLDDVRAHTKASASCGNCTGLVEQLMTLTLGDTYNPAAVQ
PMCKCTDLGHDDVRRLIKAKGLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDFPDEYA
DDYQSRYINERVHANIQKDGTYSVVPRMWGGVTNADELRAIADVVDKFEIPLVKVTGGQR
IDLLGIEKEDLPAVWADLGKAGFISGQAYAKGLRTVKTCVGEQWCRFGTQDSTGLGIRIE
KFMWGSWTPAKLKLAVSGCPRNCAEATCKDIGVICVDSGFEIHFAGAAGLDIKGTEVLGL
VHTEDEALEHIVALTQMYREQARYLERIYKWAKRIGYDEVRRQIMDDADKRKAYFDRFVF
SQKFAQVDPWSERVSGHDKHEFRPMAAIGFSEAAE