Protein Info for Atu3890 in Agrobacterium fabrum C58

Annotation: glycerol kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 TIGR01311: glycerol kinase" amino acids 4 to 493 (490 residues), 671.2 bits, see alignment E=3.6e-206 PF00370: FGGY_N" amino acids 4 to 250 (247 residues), 247.4 bits, see alignment E=1.6e-77 PF02782: FGGY_C" amino acids 260 to 449 (190 residues), 125.5 bits, see alignment E=2.4e-40

Best Hits

Swiss-Prot: 100% identical to GLPK_AGRFC: Glycerol kinase (glpK) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K00864, glycerol kinase [EC: 2.7.1.30] (inferred from 100% identity to atu:Atu3890)

Predicted SEED Role

"Glycerol kinase (EC 2.7.1.30)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or MLST (EC 2.7.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.30

Use Curated BLAST to search for 2.7.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8U940 at UniProt or InterPro

Protein Sequence (499 amino acids)

>Atu3890 glycerol kinase (Agrobacterium fabrum C58)
MGGYILAIDQGTTSTRSMVFDRDMRVIGVGQREFPQHFPASGWVEHDAEDIWKSVLETIR
LALADAGIAASDIAAIGITNQRETTVLWDRNTGEAVHRAVVWQDRRAAPVCEDLKRRGLE
PLFSKKTGLLLDPYFSGTKLKWLLDSVSGLREKAVKGEICFGTVDSFLIYRLTGGRRHVT
DATNASRTLIYNIEDNAWDDELLSILNIPRAMLPEVLDCAADFGVTDKALLGAEIPILGV
AGDQQAAVIGNACFEPGMMKSTYGTGCFALLNTGTDRVASTNRLLTTIAYRLDGVTTYAL
EGSIFIAGAAVQWLRDELGFISVASEVSALAEKADPKQRVYLVPAFTGLGAPYWDAEARG
AIFGLTRGTGPAEFARAALESVAYQTFDLLDAMRKDWAGDNGKTVLRVDGGMVASDWTMQ
RLADILAAPVDRPVFLETTILGAAWLAASRAGIWPDRKAFADHWRRDRRFSPDMEESERK
NAIAGWRDSVGRCLSKRDN