Protein Info for Atu3543 in Agrobacterium fabrum C58

Annotation: dihydroxyacetone kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K00863, dihydroxyacetone kinase [EC: 2.7.1.29] (inferred from 100% identity to atu:Atu3543)

Predicted SEED Role

"Dihydroxyacetone kinase, ATP-dependent (EC 2.7.1.29)" in subsystem Dihydroxyacetone kinases (EC 2.7.1.29)

MetaCyc Pathways

formaldehyde assimilation III (dihydroxyacetone cycle) (10/12 steps found)
glycerol degradation II (1/2 steps found)
glycerol degradation to butanol (11/16 steps found)
superpathway of glycerol degradation to 1,3-propanediol (1/4 steps found)

KEGG Metabolic Maps

Glycerolipid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CFD1 at UniProt or InterPro

Protein Sequence (564 amino acids)

>Atu3543 dihydroxyacetone kinase (Agrobacterium fabrum C58)
MKKLINDPSTVVRDMLEGIVALSPETILLQDENVVIRSGLPEAEKRKVAVLSGGGSGHEP
AHAGYVGTGMLTVAVAGDVFTSPSTDAVLAGIRAAAGPAGALVIVKNYTGDRLNFGLAAE
LARAEGIPVEIVVVADDVALKDTVPAERRRGIAGTVLVHKLAGAAAEKGLPLQEVARIAR
DAAAKLSSMGVSLGSCTLPAVGKPGFVLGETEIEVGLGIHGEQGVQRMPIASADALVQLV
IETIEADGKLAGGNRVALLVNGLGATPPMELAIVARSAVARLEAKGIVVERAWAGTFLSA
LDMPGFSLSVMQVDDAALSLIDAPTEAGAWPRGGAVNRKRVLPSANAEKTVVATNKMTAA
GERLRSGAERSARALIAAEPRLTQLDSVAGDGDLGASMVRGGEAILALPKESFGDVSDGL
MAMANAMRKAIGGSSGPFYATGLMRASRQLAGIDEPAAQQMAEAFVAAVAAVSELGGAKP
GDRTMIDALYPAAKTFRDKLVTGASAEEAWQSAVAAGEVGAEATASMKPRLGRASYLGER
AVGHPDGGAVAVGIWLKAIEAAIS