Protein Info for Atu3505 in Agrobacterium fabrum C58

Annotation: 3-hydroxyisobutyryl-CoA hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF16113: ECH_2
  • amino acids 14 to 337 (324 residues), 418.6 bits, E=2.4e-129
• PF00378: ECH_1
  • amino acids 15 to 189 (175 residues), 90 bits, E=1.7e-29

Best Hits

KEGG orthology group: K01692, enoyl-CoA hydratase [EC: 4.2.1.17] (inferred from 100% identity to atu:Atu3505)

Predicted SEED Role

"3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 3.1.2.4)

MetaCyc Pathways

• superpathway of glyoxylate cycle and fatty acid degradation (12/14 steps found)
• oleate β-oxidation (27/35 steps found)
• adipate degradation (5/5 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• adipate biosynthesis (4/5 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (7/10 steps found)
• acrylate degradation I (3/5 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• propanoyl-CoA degradation II (3/5 steps found)
• L-valine degradation I (5/8 steps found)
• fatty acid β-oxidation I (generic) (4/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• β-alanine biosynthesis II (3/6 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• valproate β-oxidation (5/9 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (2/5 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• benzoyl-CoA degradation I (aerobic) (3/7 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
• 3-phenylpropanoate degradation (4/10 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• phenylacetate degradation I (aerobic) (3/9 steps found)
• superpathway of phenylethylamine degradation (3/11 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.17

Use Curated BLAST to search for 3.1.2.4 or 4.2.1.17

Sequence Analysis Tools

See Q7CSM7 at UniProt or InterPro

Protein Sequence (350 amino acids)

>Atu3505 3-hydroxyisobutyryl-CoA hydrolase (Agrobacterium fabrum C58)
METEVVVETKGAGGIIGLNRPKAINSLTLPMVRALFQALQRFEDDPAISCVVLRGEGERG
LCAGGDVRIIHDLGKAGDPQVLDFWQEEFPLNYRIARFGKPYVALMDGIVMGGGVGISAH
GSHRIVTERTRLAMPETGIGYFPDVGGSWLLPRAPGECGTWLGLTGNAVTAADAIYAGFA
DYCVPSGRLDALVQALSKATDIEGVDAAIASYATDGGEGLLAANRDIIDATFRFDTVEEI
FAALSARDDAFSRETLEILRKRSPTSLKLTLKLLRLGRESASLIECLEREFAAGTEILRQ
HDFYEGVRAALVDKDRNPRWRPARLEEVSEEDLAPYFAAHSGNLFPEHRL