Protein Info for Atu3426 in Agrobacterium fabrum C58

Annotation: sulfonate monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 389 PF00296: Bac_luciferase" amino acids 9 to 332 (324 residues), 251.3 bits, see alignment E=7.6e-79 TIGR03565: alkanesulfonate monooxygenase, FMNH(2)-dependent" amino acids 9 to 355 (347 residues), 588.3 bits, see alignment E=2.3e-181

Best Hits

Swiss-Prot: 100% identical to SSUD_AGRFC: Alkanesulfonate monooxygenase (ssuD) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K04091, alkanesulfonate monooxygenase [EC: 1.14.14.5] (inferred from 100% identity to atu:Atu3426)

MetaCyc: 60% identical to alkanesulfonate monooxygenase subunit (Pseudomonas putida DS1)
RXN-18086 [EC: 1.14.14.34]

Predicted SEED Role

"Alkanesulfonate monooxygenase (EC 1.14.14.5)" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization (EC 1.14.14.5)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.14.14.5

Use Curated BLAST to search for 1.14.14.34 or 1.14.14.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UAE8 at UniProt or InterPro

Protein Sequence (389 amino acids)

>Atu3426 sulfonate monooxygenase (Agrobacterium fabrum C58)
MSKHDKPLDFLWFIPTSGDGSYLGSDDLSRPADPGYFREIAQAADRLGYSGVLIPTGVAC
EESFILAANLAAYTEKLKFLVAIRPGVASPAYYARLASTLDRVSHGRLLLNIVVGGSAQE
LAGDGIFLPHDERYDHADEFFQVFNSLIETGKGDLDGKYIKAQGARLGLPPVQEPRPPLY
FGGSSDAGIEFSAGITDKYLTWGEPPAQVAEKIVKVRAAAAKNGREVTFGIRLHFIVRET
DEEAWADADRLISKLSDETIASAQEVFSKASDSVGQARMQALHNGRRDKLEVSPNLWAGI
GLVRSGAGTALVGSPKTVAARLREYQALGIDTVIASGYPHLEEAYRLSELLFPEIGIDGP
RNQIRSSFGAKQVFGGGGHGGNVKLVSGS