Protein Info for Atu3353 in Agrobacterium fabrum C58
Annotation: mannose-1-phosphate guanylyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 46% identical to MANC_SALTY: Mannose-1-phosphate guanylyltransferase ManC (manC) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] (inferred from 100% identity to atu:Atu3353)MetaCyc: 47% identical to phosphomannose isomerase / GDP-D-mannose pyrophosphorylase (Pseudomonas aeruginosa)
Mannose-6-phosphate isomerase. [EC: 5.3.1.8]; Mannose-1-phosphate guanylyltransferase. [EC: 5.3.1.8, 2.7.7.13]
Predicted SEED Role
No annotation
MetaCyc Pathways
- colanic acid building blocks biosynthesis (11/11 steps found)
- GDP-mannose biosynthesis (4/4 steps found)
- β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
- mannitol biosynthesis (2/3 steps found)
- D-mannose degradation I (1/2 steps found)
- D-mannose degradation II (1/2 steps found)
- 1,5-anhydrofructose degradation (3/5 steps found)
- mannitol degradation II (2/4 steps found)
- superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)
- β-(1,4)-mannan degradation (2/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 5.3.1.8
Use Curated BLAST to search for 2.7.7.13 or 2.7.7.22 or 5.3.1.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A9CF23 at UniProt or InterPro
Protein Sequence (476 amino acids)
>Atu3353 mannose-1-phosphate guanylyltransferase (Agrobacterium fabrum C58) MNEQSRKITPVLLAGGAGSRLWPVSRDQLPKQFQPLVGNLSTYQQTLKRVGDKSLYSEPL VITNEDFRFFARRQAEEIDLPATVVLEPARRDSAAAMAAAAVLAERREPGCLVLALAADH VVLDVDKFSEAVKLGAKAADQGNIVVFGLVPTEPRTSYGYIKPGDAIDGEKDLSTVDAFV EKPDVTTAISYLEKGYLWNSGNFLFRSDVMIAELKAFAPEILAAVTQAVEQYESDLGFVR LHQASFEASPKNSIDYAVIEKTKRMAVVHGHFRWSDIGSWDAIWEIADKHDNDNALEGDG VFIDSEGCLIHSTQLLTTVVGAKDLVVVATKDAVLVVPKNRVQDVKGLVDTLKGGDHAPQ TQTHKRVYRPWGYIEHMYVDQRYRVGHITVDPGHRISFQKHYHRSEHWIVVKGTATVTIG EETRLLTENQSVYIPIGQPHRLANEGQIPLELVEIQTGAYLGEDDVIRIEDDYKRQ