Protein Info for Atu3251 in Agrobacterium fabrum C58

Annotation: haloacid dehalogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 228 PF00702: Hydrolase" amino acids 17 to 184 (168 residues), 95 bits, see alignment E=1.6e-30 PF13419: HAD_2" amino acids 20 to 189 (170 residues), 77.7 bits, see alignment E=2.4e-25 PF12710: HAD" amino acids 20 to 156 (137 residues), 34 bits, see alignment E=8e-12 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 95 to 190 (96 residues), 69.1 bits, see alignment E=5e-23 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 96 to 169 (74 residues), 28.7 bits, see alignment E=1.6e-10

Best Hits

Swiss-Prot: 41% identical to HXPA_ECOLI: Hexitol phosphatase A (hxpA) from Escherichia coli (strain K12)

KEGG orthology group: K01112, [EC: 3.1.3.-] (inferred from 100% identity to atu:Atu3251)

MetaCyc: 41% identical to hexitol phosphatase A (Escherichia coli K-12 substr. MG1655)
Sorbitol-6-phosphatase. [EC: 3.1.3.50]; Sugar-phosphatase. [EC: 3.1.3.50, 3.1.3.23]; Mannitol-1-phosphatase. [EC: 3.1.3.50, 3.1.3.23, 3.1.3.22]; Glycerol-1-phosphatase. [EC: 3.1.3.50, 3.1.3.23, 3.1.3.22, 3.1.3.21]

Predicted SEED Role

"Putative phosphatase YfbT" in subsystem 2-phosphoglycolate salvage

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.- or 3.1.3.21 or 3.1.3.22 or 3.1.3.23 or 3.1.3.50

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CEX3 at UniProt or InterPro

Protein Sequence (228 amino acids)

>Atu3251 haloacid dehalogenase (Agrobacterium fabrum C58)
MHSKVPPQTQIFSQEFAAFLFDMDGTLVNSIAVVERVWREWAVANGIEPNAFLQRIHGMR
ASEVIRREAVPGLDVDAEADMILRAEMDDVEGIIPIPGAADFLASLPAGKWAIVTSAVRE
LAEKRLAAAGITPPPVMICADDVVNGKPDPEGYRKAAERLGVAPESCVVFEDAPAGIQAG
ERMGAAVVVINVTHSHPIETPHLAIGSYGELDVVIDGNGGLKLTSSKA