Protein Info for Atu2798 in Agrobacterium fabrum C58

Annotation: precorrin-6y methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 419 PF00590: TP_methylase" amino acids 21 to 203 (183 residues), 68.8 bits, see alignment E=6.6e-23 TIGR02467: precorrin-6y C5,15-methyltransferase (decarboxylating), CbiE subunit" amino acids 23 to 217 (195 residues), 150.1 bits, see alignment E=5.8e-48 TIGR02469: precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit" amino acids 248 to 369 (122 residues), 117.6 bits, see alignment E=4e-38 PF01135: PCMT" amino acids 249 to 366 (118 residues), 25.4 bits, see alignment E=1.1e-09

Best Hits

Swiss-Prot: 64% identical to COBL_SINSX: Precorrin-6Y C(5,15)-methyltransferase [decarboxylating] (cobL) from Sinorhizobium sp.

KEGG orthology group: K00595, precorrin-6Y C5,15-methyltransferase / precorrin-8W decarboxylase [EC: 1.-.-.- 2.1.1.132] (inferred from 100% identity to atu:Atu2798)

MetaCyc: 64% identical to precorrin-6B (C5,15) methyltransferase subunit (Pseudomonas denitrificans (nom. rej.))
Precorrin-6Y C(5,15)-methyltransferase (decarboxylating). [EC: 2.1.1.132]

Predicted SEED Role

"Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-) / Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-)" in subsystem Coenzyme B12 biosynthesis (EC 2.1.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-, 2.1.1.-

Use Curated BLAST to search for 1.-.-.- or 2.1.1.- or 2.1.1.132

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CW86 at UniProt or InterPro

Protein Sequence (419 amino acids)

>Atu2798 precorrin-6y methyltransferase (Agrobacterium fabrum C58)
MTSEYSGTAKASTEKNEKPWLSIVGIGEDGLQGLGENARQAIEAAEVVFGGKRHLELAAG
AIRGEARAWPVPFDVDMNDVLALRGRLVCVLASGDPFFYGAGVTLLRRIPAEETACYPSP
SAFSLAASRLGWALQSAECVSLHGRSIDLLRPHLHHGARIIALTSDERDPALIARLLSDA
GFGRSEFILLEALGGDREKRRQIRAADFGFEDTDPLNVVAIEVVADEGARLLPFAHGLDD
GLFEHDGQITKREIRAVTLSSLASRRGELLWDIGAGSGSIGIEWMLSHPSLRAIAIEQHP
ERAERIARNADAFGVPGLEVMIGTAPAAFEGLSSPDAIFIGGGGSETGVLEAAMDALKPG
GRLVANAVTLEMEAVLLAAHAKFGGSMIRLEVSRAAAVGSMSGWRPAMPVTQWSWVKPW