Protein Info for Atu2694 in Agrobacterium fabrum C58

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 424 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 4 to 390 (387 residues), 331.2 bits, see alignment E=9.3e-103 PF00919: UPF0004" amino acids 4 to 90 (87 residues), 67.4 bits, see alignment E=9.1e-23 TIGR01579: MiaB-like tRNA modifying enzyme" amino acids 7 to 390 (384 residues), 436.4 bits, see alignment E=1.4e-134 PF04055: Radical_SAM" amino acids 141 to 313 (173 residues), 78.6 bits, see alignment E=6.4e-26

Best Hits

Swiss-Prot: 57% identical to MTAB_RICPR: Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB (mtaB) from Rickettsia prowazekii (strain Madrid E)

KEGG orthology group: None (inferred from 100% identity to atu:Atu2694)

Predicted SEED Role

"tRNA-t(6)A37 methylthiotransferase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CWG5 at UniProt or InterPro

Protein Sequence (424 amino acids)

>Atu2694 hypothetical protein (Agrobacterium fabrum C58)
MSGVEVITFGCRLNTYESEVMRAEAEKAGLNNAVLVNTCAVTGEAVRQARQAIRRARRDN
PHARIIVTGCAAQTEKQTFADMPEVDAVLGNEEKLKSASYRALPDFGVSAEEKLRVNDIM
SIKATAPQMVKHIDGHVRAFIQVQNGCDHRCTFCIIPYGRGNSRSVPMGAVVDQARRLTE
SGYCEIVLTGVDATSYGADLPGEPSLGYLAKTLLKQVPDIIRLRLSSIDSIEADHHLMDL
IADEPRFMPHLHLSLQHGDDMILKRMKRRHLRADAIRFCNEVRSLRPDIAFGADMIAGFP
TETEEMLENAATLAEECGISSLHVFPYSPREGTPAVRMPQLDRALVKERAGRLRARGEAL
KQAHLQNMVGSVQTVLVENSGFAHTDNFTLVAAPDLAPRTLAQVAITGHNGKHLNMKLLA
ADAA