Protein Info for Atu2493 in Agrobacterium fabrum C58

Annotation: NAD(P)+ transhydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 524 transmembrane" amino acids 171 to 194 (24 residues), see Phobius details amino acids 416 to 434 (19 residues), see Phobius details amino acids 440 to 458 (19 residues), see Phobius details amino acids 466 to 485 (20 residues), see Phobius details amino acids 491 to 513 (23 residues), see Phobius details TIGR00561: NAD(P)(+) transhydrogenase (AB-specific), alpha subunit" amino acids 2 to 523 (522 residues), 683.2 bits, see alignment E=1.1e-209 PF05222: AlaDh_PNT_N" amino acids 4 to 139 (136 residues), 160.3 bits, see alignment E=4.6e-51 PF01262: AlaDh_PNT_C" amino acids 143 to 373 (231 residues), 228.7 bits, see alignment E=8.1e-72 PF12769: PNTB_4TM" amino acids 440 to 523 (84 residues), 115.1 bits, see alignment E=2.4e-37

Best Hits

Swiss-Prot: 81% identical to PNTA_RHOSH: NAD(P) transhydrogenase subunit alpha (pntA) from Rhodobacter sphaeroides

KEGG orthology group: K00324, NAD(P) transhydrogenase subunit alpha [EC: 1.6.1.2] (inferred from 100% identity to atu:Atu2493)

Predicted SEED Role

"NAD(P) transhydrogenase alpha subunit (EC 1.6.1.2)" in subsystem Phosphate metabolism (EC 1.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.1.2

Use Curated BLAST to search for 1.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CHR5 at UniProt or InterPro

Protein Sequence (524 amino acids)

>Atu2493 NAD(P)+ transhydrogenase (Agrobacterium fabrum C58)
MRIGTPKELYEGEARVGMTPDSAQALQKLGYDCVIEAGAGKAAGFSDDAYRAAGVTVVDS
ADALFAGSDIIAKVRPPETSEIDRLSSDKTLISFFYPAQNKDLLEQAKEKGANVIAMDMV
PRISRAQKMDALSSMANIAGYRAVIEAGNNFGRFFTGQVTAAGKVPPAKVLVIGAGVAGL
AAIGTATSLGAITYAFDVRPEVAEQIESMGAEFVYLDFADQQQDGAATGGYAAPSSPEFR
EKQLEKFRELAPQIDIVITTALIPGRDAPKLWLADMVAAMKPGSVIIDLAAERGGNCDLT
VPDQRIVSDNGVIVIGYTDFPSRMAAQASTLYSTNIRHMMTDLTPAKDGKPVHNMEDDVI
RGATVTYQGEITFPPPPPKIQAIAAQKPKERVKELTPEEKHAKERAEFKAQTKSQVGLLV
IGTAALLLVGLFAPASFMSHFIVFVLACFIGFQVIWNVSHSLHTPLMAVTNAISGIVIIG
ALLQIGSGNWLVVILASLSVLIATINIVGGFLVTRRMLAMFQKS