Protein Info for Atu2465 in Agrobacterium fabrum C58

Annotation: two component sensor kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 464 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details transmembrane" amino acids 29 to 29 (1 residues), see Phobius details amino acids 168 to 190 (23 residues), see Phobius details PF08521: 2CSK_N" amino acids 22 to 163 (142 residues), 137.9 bits, see alignment E=3.7e-44 PF00512: HisKA" amino acids 240 to 303 (64 residues), 28.1 bits, see alignment E=2.7e-10 PF02518: HATPase_c" amino acids 354 to 456 (103 residues), 72.5 bits, see alignment E=5.8e-24

Best Hits

KEGG orthology group: K07649, two-component system, OmpR family, sensor histidine kinase TctE [EC: 2.7.13.3] (inferred from 100% identity to atu:Atu2465)

Predicted SEED Role

"Tricarboxylate transport sensor protein TctE"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CHT1 at UniProt or InterPro

Protein Sequence (464 amino acids)

>Atu2465 two component sensor kinase (Agrobacterium fabrum C58)
MRQAAYSLRRRLLGWLLISTAIIGCVALTDTWREAVNTANVVSDRVLSGSALAIAERVVV
AEDGSLEVDIPYVALEMLTSAAQDRVFYRVDGPNGQFLTGYQTLPTVENTNGDTPAYRDA
VFRDEPIRIAAIRRFASTGINSVPFSVTVAETTIARSQLAQAIILRSALRLLLMIVGAAI
IVWIAVTISLRPLYRLSEAIAERSPNDLHPIRQSVPVEVENLVETVNSFMVRLQSALDAL
RHFTGNASHQLRTPLAIISTQLALSARAASLEEAQAAALKGGASVAHAEHILAQLLRMAN
IDAAGSSEKHDFSAIDLVAVAQNVTADFVPRAADAGIDLGFEGEGEATISAEPLLIGELI
GNLVSNAINYAGRGAEVTVRVGGPAGAVWLEVEDNGPGIPPEKRAMVRQRFARGEANAAP
GAGLGLAIIEEIAGLFGGRLTLEDGADGRGLKARVVFAAPERAA