Protein Info for Atu2386 in Agrobacterium fabrum C58

Annotation: dihydropyrimidinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02033: dihydropyrimidinase
  • amino acids 5 to 453 (449 residues), 601.5 bits, E=5.6e-185
• PF01979: Amidohydro_1
  • amino acids 48 to 419 (372 residues), 81.4 bits, E=7.6e-27

Best Hits

Swiss-Prot: 53% identical to HYDA_PSEAE: D-hydantoinase/dihydropyrimidinase (dht) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01464, dihydropyrimidinase [EC: 3.5.2.2] (inferred from 100% identity to atu:Atu2386)

MetaCyc: 49% identical to Dihydropyrimidinase (Homo sapiens)
Dihydropyrimidinase. [EC: 3.5.2.2]; 3.5.2.2 [EC: 3.5.2.2]; 3.5.2.2 [EC: 3.5.2.2]

Predicted SEED Role

"Dihydropyrimidinase (EC 3.5.2.2)" in subsystem Hydantoin metabolism or Pyrimidine utilization (EC 3.5.2.2)

MetaCyc Pathways

• superpathway of pyrimidine ribonucleosides degradation (4/5 steps found)
• thymine degradation (2/3 steps found)
• uracil degradation I (reductive) (2/3 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pantothenate and CoA biosynthesis
• Pyrimidine metabolism
• beta-Alanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.2

Sequence Analysis Tools

See Q7CX67 at UniProt or InterPro

Protein Sequence (485 amino acids)

>Atu2386 dihydropyrimidinase (Agrobacterium fabrum C58)
MIATIIKNGTIVTADLTYKADIRIEGGRITEIGPDLTGGTILDATECYIMPGGIDPHVHL
EMPFMGTYSADDFESGTRAALAGGTTMVVDFCLPDPGQSLLDALQRWDNKATRANCDYSF
HMAVTWWGERVFNEMKTVVQEKGINSFKHFMAYKGALMVNDDEMFASFSRCAELGAIPFV
HAENGDIVAQMQEKLMAEGNVGPEAHAYSRPPSVEGEATNRAIIIADMAGAPLYVVHTSC
EQAHEAIRRARQNGMRVYGEPLIQHLILDESEYANADWDHAARRVMSPPFRNRQHQDSLW
AGLASGSLQCVATDHCAFTTEQKRFGLGDFRKIPNGTGGLEDRMPLLWTHGVATGRLTMN
EFVAVTSTNIAKILNIYPRKGAILVGSDADIVVWDPALEKTISAASQQSAIDYNVFEGQK
VKGLPRYTLSRGLVSVEEGTIETQEGHGQFVARDPYPAVSRALSTWKELVSPRKVERTGI
PASGV