Protein Info for Atu2385 in Agrobacterium fabrum C58

Annotation: N-carbamoyl-beta-alanine amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 409 TIGR01879: amidase, hydantoinase/carbamoylase family" amino acids 6 to 403 (398 residues), 391.8 bits, see alignment E=1.7e-121 PF01546: Peptidase_M20" amino acids 77 to 402 (326 residues), 103 bits, see alignment E=2.2e-33 PF07687: M20_dimer" amino acids 208 to 303 (96 residues), 27.5 bits, see alignment E=2.6e-10

Best Hits

Swiss-Prot: 82% identical to HYUC_RHIML: N-carbamoyl-L-amino-acid hydrolase (hyuC) from Rhizobium meliloti

KEGG orthology group: K01431, beta-ureidopropionase [EC: 3.5.1.6] (inferred from 100% identity to atu:Atu2385)

MetaCyc: 82% identical to N-carbamoyl-L-amino-acid hydrolase subunit (Sinorhizobium meliloti CECT4114)
N-carbamoyl-L-amino-acid hydrolase. [EC: 3.5.1.87]

Predicted SEED Role

"Beta-ureidopropionase (EC 3.5.1.6)" in subsystem Hydantoin metabolism or L-2-amino-thiazoline-4-carboxylic acid-Lcysteine conversion or Pyrimidine utilization (EC 3.5.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.6

Use Curated BLAST to search for 3.5.1.6 or 3.5.1.87

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CX68 at UniProt or InterPro

Protein Sequence (409 amino acids)

>Atu2385 N-carbamoyl-beta-alanine amidohydrolase (Agrobacterium fabrum C58)
MTVNGDRLWDSLMDMAKIGPGIAGGNNRRTLTDEDAEGRSLFKSWCEAAGLTMGVDRMGT
MFATRPGEDPDALPVYIGSHLDTQPTGGKFDGVLGVLAGLEVVRSLNDLNIKTKHPIVVT
NWSNEEGARFAPAMLASGVFAGIHDLDYAYSRTDTDGKTYGDELKRIGWLGEEEVGARRM
HAYFEYHIEQGPILEAEGKQIGVVTHGQGLWWLEVTLTGKEAHTGSTPMAMRVNAGLAAA
RILEKVQEVAMAHQPGAVAGVGQMIFTPNSRNVLPGKVVFTIDLRTPSQAKLDNMRAIFE
REVPVIAEELGVGCSIEAIGHFDPVTFDPVLVGRVRSAAERLGYTHMDIISGAGHDACWT
ARVAPSTMIFCPCVGGLSHNEAEEISPEWAAAGCDVLLHAVLETAEIVQ