Protein Info for Atu1850 in Agrobacterium fabrum C58
Annotation: phosphoribosylformylglycinamidine synthetase II
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to PURL_AGRFC: Phosphoribosylformylglycinamidine synthase subunit PurL (purL) from Agrobacterium fabrum (strain C58 / ATCC 33970)
KEGG orthology group: K01952, phosphoribosylformylglycinamidine synthase [EC: 6.3.5.3] (inferred from 100% identity to atu:Atu1850)Predicted SEED Role
"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
- superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
- superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
- 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
- superpathway of 5-aminoimidazole ribonucleotide biosynthesis (5/6 steps found)
- 5-aminoimidazole ribonucleotide biosynthesis II (4/5 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.3
Use Curated BLAST to search for 6.3.5.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8UEB0 at UniProt or InterPro
Protein Sequence (744 amino acids)
>Atu1850 phosphoribosylformylglycinamidine synthetase II (Agrobacterium fabrum C58) MSISNSIKITPELVASHGLKPDEYQRILDLIGREPSFTELGIFSAMWNEHCSYKSSKKWL KTLPTTGPRVIQGPGENAGVVDIDDGDCVVFKMESHNHPSYIEPYQGAATGVGGILRDVF TMGARPIAAMNALRFGAPDHPKTRHLVAGVVAGVGGYGNSFGVPTVGGEVEFDPRYNGNI LVNAFAAGLAKSNAIFLSEAKGVGLPVVYLGAKTGRDGVGGATMASAEFDESIEEKRPTV QVGDPFTEKCLLEACLELMKTGAVIAIQDMGAAGLTCSAVEMGAKGDLGIELDLNAVPVR EERMTAYEMMLSESQERMLMVLEPSKEEVAKAIFVKWGLDFAIVGKTTDDLRFRVLHNGE EVANLPIKELGDEAPEYDRPWTPAKVPAALSETDIPEADIADALVSLVGSANNSSRRWVY EQYDTLIQGNSLQLPGGDAGVVRVEGHDKKALAFSSDVTPRYVEADAFEGGKQAVAECWR NITATGALPLAATDNLNFGNPEKPEIMSQLVHAIKGIGEACRVLEFPIVSGNVSLYNETN GQAILPTPTIGGVGLLKDWGRMARIRFAAADEVVLLVGAPAGLGTHIAQSVYMRDVHGRT DGPAPHVDLIAEKKNGDFVRGLITEGLTTAVHDCSSGGLALAVAEMAISSGIGATIDAVE GHNPILTFYGEDQARYVLTVKKSDLDKVRAAAKAAGVSCPLIGTTGGSTVKLGTARAVEI KELHLAYESWFPQFMDGETLIAAE