Protein Info for Atu1787 in Agrobacterium fabrum C58

Annotation: UDP-N-acetylglucosamine pyrophosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 7 to 434 (428 residues), 469.7 bits, E=5e-145
• PF00483: NTP_transferase
  • amino acids 7 to 168 (162 residues), 43.6 bits, E=6.9e-15
• PF12804: NTP_transf_3
  • amino acids 7 to 146 (140 residues), 79.7 bits, E=7.4e-26
• PF02348: CTP_transf_3
  • amino acids 7 to 167 (161 residues), 35.9 bits, E=1.8e-12
• PF00132: Hexapep
  • amino acids 267 to 301 (35 residues), 34.5 bits, 2.7e-12
  • amino acids 380 to 413 (34 residues), 37.1 bits, 4.2e-13
• PF14602: Hexapep_2
  • amino acids 380 to 413 (34 residues), 30.8 bits, 4.8e-11

Best Hits

Swiss-Prot: 100% identical to GLMU_AGRFC: Bifunctional protein GlmU (glmU) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 100% identity to atu:Atu1787)

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (11/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• peptidoglycan recycling I (8/14 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (6/24 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See Q8UEH0 at UniProt or InterPro

Protein Sequence (453 amino acids)

>Atu1787 UDP-N-acetylglucosamine pyrophosphorylase (Agrobacterium fabrum C58)
MERSSLAVILAAGDSTRMKSSKSKVLHPVAGRPMIGHVVEAVAGAGVGAVALVVGRDADN
VAAAASLKGLQVEAFLQKERKGTGHAVLAAREAIKRGFDDVIVAYGDVPLITSATLDRAR
EAIAAGADVAVIGFHTDRPTGYGRLLVENGELVAIREEKDATDEERKVTWCNSGLMAING
RNALDLLDRIGNSNVKGEYYLTDVVEIARSLGRRAIAIDAPEKELTGCNNRAELAFIERL
WQERRRHELMVDGVSMIAPETVFLSFDTKIGQDVLIEPNVVFGPGVTIEPGAIVHAFSHL
EGAHLAEGAVVGPFARLRPGANLHANAKVGNFCEVKKAEIGEGAKVNHLTYIGDAFVGAG
SNIGAGAITCNYDGYNKSETRIGANSFIGSNSSLVAPVTIGERAYIASGSVITDDVPADA
LAFGRARQEVKPGRAVALRERAKAQKEAKKKSS