Protein Info for Atu1701 in Agrobacterium fabrum C58

Annotation: protein-L-isoaspartate O-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 213 PF01135: PCMT" amino acids 9 to 208 (200 residues), 133.1 bits, see alignment E=4.4e-42 PF00398: RrnaAD" amino acids 62 to 132 (71 residues), 33.9 bits, see alignment E=6.5e-12 PF08704: GCD14" amino acids 64 to 127 (64 residues), 26.1 bits, see alignment E=2.2e-09 PF02353: CMAS" amino acids 68 to 211 (144 residues), 30 bits, see alignment E=1.1e-10 PF13847: Methyltransf_31" amino acids 75 to 156 (82 residues), 30.2 bits, see alignment E=1.2e-10 PF13649: Methyltransf_25" amino acids 80 to 156 (77 residues), 31.5 bits, see alignment E=8.3e-11 PF08241: Methyltransf_11" amino acids 81 to 156 (76 residues), 28.4 bits, see alignment E=7.4e-10

Best Hits

Swiss-Prot: 55% identical to PIMT_RHIME: Protein-L-isoaspartate O-methyltransferase (pcm) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00573, protein-L-isoaspartate(D-aspartate) O-methyltransferase [EC: 2.1.1.77] (inferred from 100% identity to atu:Atu1701)

Predicted SEED Role

"Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)" in subsystem Ton and Tol transport systems (EC 2.1.1.77)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.77

Use Curated BLAST to search for 2.1.1.77

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CYQ2 at UniProt or InterPro

Protein Sequence (213 amino acids)

>Atu1701 protein-L-isoaspartate O-methyltransferase (Agrobacterium fabrum C58)
MVEKEGFAALVLRLRGEGISDLDLLTAVEQTPRSKFVPPQFAADAYSSRTIPIDCGSFME
GADMAVKILARLQLKPGQRVLEIGTGSGFMTAIIARRVERVFSLERYKTLVQQAQNCLDD
LSIRNVVIRQADGSNGLVGEGTFDRIVSTAAFTTMPRFFAEQIVSGGMMIAPIILEDERC
VMTRFSKTGSRFEKEELFETPYLPLGSHIARHL