Protein Info for Atu1548 in Agrobacterium fabrum C58

Annotation: formate dehydrogenase H alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01701: oxidoreductase alpha (molybdopterin) subunit
  • amino acids 36 to 781 (746 residues), 947.1 bits, E=3.2e-289
• PF00384: Molybdopterin
  • amino acids 132 to 515 (384 residues), 75.2 bits, E=5.3e-25
• PF01568: Molydop_binding
  • amino acids 670 to 754 (85 residues), 32.2 bits, E=9.7e-12

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 100% identity to atu:Atu1548)

Predicted SEED Role

"Putative formate dehydrogenase oxidoreductase protein" in subsystem Formate hydrogenase

MetaCyc Pathways

• formate oxidation to CO₂ (1/1 steps found)
• oxalate degradation VI (1/4 steps found)
• oxalate degradation III (1/5 steps found)
• purine nucleobases degradation II (anaerobic) (14/24 steps found)
• superpathway of C1 compounds oxidation to CO₂ (5/12 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

See A9CIY0 at UniProt or InterPro

Protein Sequence (788 amino acids)

>Atu1548 formate dehydrogenase H alpha subunit (Agrobacterium fabrum C58)
MFLTNGKRRQPGTRRQFPGKRTMSKTDFIGKASTAAGGWGALKSVGKRLLESGAPISGAR
TLLKTNQPDGFDCPGCAWGDPEHGSSFEFCENGVKAVSWEATEARVPPDFFARHTVSELR
SWSDYDLEKQGRLTHPMRYDRASDTYLPVSWDDAFAEIGRILNSLDSPDRAEFYTSGRAS
NEAAFLYQLMVRLYGTNNFPDCSNMCHEASGVGLNASIGVGKGTVLLEDFEQTDAIFVIG
QNPGTNHPRMLGDLRRAALRGARIAVFNPIREKGLERFADPQDKIEMITGSSTKIATNYY
QPRQGGDMAAVRGMSKAVFAADDAARAAGEPAIIDYDFIADHAAEFEAYRAAVDATSWES
ILDQSGLTRAEIEEAAGIYMNAGSVIATWAMGVTQHRHSVLIVREIANFMLLRGNVGRPG
AGLCPVRGHSNVQGDRTVGIDEKAPPALLDALEKELGVPMPRKPGHNTVEAVAAMLDGKA
QTFIALGGNFLRATPDSPLIVKAFEKQKLTVNITTKLNHSHLVPGETSFVLPCLGRTEID
RNSAGRSQIVTVEDSMSMVHGSGGINPPASDALRSEVAIVAGIAEATLGNVNVNWKALAD
DYDLIRDMIERVIPGFDNFNERVRIPRGFHLRNAAAERQWNTPAKKATFYSGPLPEKTEH
QQALTRDNLFVLQTFRSHDQYNTTIYGMDDRYRGVYGERQVIFMNPADMEALGAHSRQRV
DVIGEYGDGVERIARNFRLVPYNIPRGSVGGYYPELNVLVPLSSYGEGSFTPTSKSVLVS
VRLLADAR