Protein Info for Atu1526 in Agrobacterium fabrum C58

Annotation: 6-phosphogluconate dehydrogenase, decarboxylating

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 transmembrane" amino acids 410 to 431 (22 residues), see Phobius details TIGR00873: 6-phosphogluconate dehydrogenase (decarboxylating)" amino acids 5 to 461 (457 residues), 630.1 bits, see alignment E=1.2e-193 PF03446: NAD_binding_2" amino acids 5 to 170 (166 residues), 162.4 bits, see alignment E=1.4e-51 PF03807: F420_oxidored" amino acids 6 to 53 (48 residues), 21.8 bits, see alignment 3.5e-08 PF00393: 6PGD" amino acids 180 to 469 (290 residues), 393.8 bits, see alignment E=7.9e-122

Best Hits

Swiss-Prot: 50% identical to 6PGD_SYNE7: 6-phosphogluconate dehydrogenase, decarboxylating (gnd) from Synechococcus elongatus (strain PCC 7942)

KEGG orthology group: K00033, 6-phosphogluconate dehydrogenase [EC: 1.1.1.44] (inferred from 100% identity to atu:Atu1526)

MetaCyc: 49% identical to 6-phosphogluconate dehydrogenase (decarboxylating) (Bacillus subtilis)
Phosphogluconate dehydrogenase (decarboxylating). [EC: 1.1.1.44]

Predicted SEED Role

"6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)" in subsystem Pentose phosphate pathway (EC 1.1.1.44)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.44

Use Curated BLAST to search for 1.1.1.44

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CIZ2 at UniProt or InterPro

Protein Sequence (476 amino acids)

>Atu1526 6-phosphogluconate dehydrogenase, decarboxylating (Agrobacterium fabrum C58)
MEQAEIGLIGLGVMGSNLALNIAEKGNKIAVFNRTPEVTRKFYADAGELQGQIIPCETIE
EFVAAIRPPRPIIIMIKAGDPVDQQMEILKPHLANGDIMIDAGNANFRDTIRRFDNLKDS
GLTFIGMGVSGGEEGARHGPSIMVGGTEDSWKRVEKVLTSISAKYNDDPCVAWLGNDGAG
HFVKTIHNGIEYADMQMIAEIYGILRDGLKMSAVEIADVFAEWNKGRLNSYLIEITEKVL
RAADPITGKPMVDLILDKAGQKGTGKWSVIEAQNMGVAATAIEAAVAARILSSQKDEREA
AEKIFGLPTLAAAPADKKAFIADLESALLAAKVGAYAQGFAIMSAASKEFNWNLPMPTIA
RIWRAGCIIRSEFLDEITSAFTKDPHVANLIVTPAFSAIVKDTDAPLRRVVSYAVLSGLP
VSALASALGYFDAYRRGRGSANLIQAQRDFFGAHGFERTDGVDKPHGPWGSGADIF