Protein Info for Atu1462 in Agrobacterium fabrum C58

Annotation: glycine cleavage system protein P2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 954 transmembrane" amino acids 334 to 353 (20 residues), see Phobius details PF02347: GDC-P" amino acids 20 to 444 (425 residues), 603.1 bits, see alignment E=3e-185 amino acids 460 to 732 (273 residues), 33 bits, see alignment E=3.2e-12 TIGR00461: glycine dehydrogenase" amino acids 21 to 943 (923 residues), 1442.3 bits, see alignment E=0 PF21478: GcvP2_C" amino acids 772 to 893 (122 residues), 197.2 bits, see alignment E=7.1e-63

Best Hits

Swiss-Prot: 100% identical to GCSP_AGRFC: Glycine dehydrogenase (decarboxylating) (gcvP) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K00281, glycine dehydrogenase [EC: 1.4.4.2] (inferred from 100% identity to atu:Atu1462)

Predicted SEED Role

"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UFD6 at UniProt or InterPro

Protein Sequence (954 amino acids)

>Atu1462 glycine cleavage system protein P2 (Agrobacterium fabrum C58)
MTTPTEFHFTDYQPYDFANRRHIGPSPSEMAEMLKVVGYKSLDALIDATVPSSIRQKVPL
TWGAALTEREALDRLRETANKNQVLTSLIGQGYYGTITPPVIQRNILENPAWYTAYTPYQ
PEISQGRLEALLNYQTMVCDLTGLDVANASLLDEATAAAEAMAMCQRVAKSKATAFFVDA
NCHPQTIALIETRAAPLGWKVIIGNPFTDLDPVDVFGAIFQYPGTHGHVSDFTGLISRLH
QTGAIAAVAADLLALTLLKSPGEMGADIAIGTSQRFGVPVGYGGPHAAYMSVKDAHKRSM
PGRLVGVSVDARGNRAYRLSLQTREQHIRREKATSNICTAQVLLAVMASMYGVFHGPQGI
KAIAQQTHQKAVLMAKGLEKLGYTIEPETFFDTITVEVGHMQGVILRSAVAEGVNLRKVG
ATKIGMSLDERTRPATLEAVWRAFGGNFSISDFEPDYRLPKDLLRTSQYMTHPIFHMNRA
ESEMTRYIRRLSDRDLALDRSMIPLGSCTMKLNATAEMLPITWPEFSDIHPFVPANQALG
YKEMIDDLSEKLCSVTGYDAFSMQPNSGAQGEYAGLLTIRNYHLANGGTHRDVCLIPTSA
HGTNPASAQMVGMKVVPVKVRDNGDIDIDDFRLKAEQYAENLSCCMITYPSTHGVFEETV
REICEITHKHGGQVYLDGANMNAMVGLARPGDIGSDVSHLNLHKTFCIPHGGGGPGMGPI
GVKAHLAPFLPGHPTTDGREGAVSAAPFGSPSILPISWSYCLMMGGEGLTQATKVAILNA
NYIAERLKGAYDVLYKSETGRVAHECIIDTRPLADSCGVTVDDVAKRLIDCGFHAPTMSW
PVAGTLMIEPTESETKAEIDRFCDAMLAIREEARDIEEGRADKNNNPLKNAPHTVEDLVG
EWDRPYSREKGCFPPGAFRIDKYWSPVNRIDNVYGDRNLICTCPPMEAYAEAAE