Protein Info for Atu1454 in Agrobacterium fabrum C58

Annotation: siroheme synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 TIGR01470: siroheme synthase, N-terminal domain" amino acids 17 to 218 (202 residues), 205.8 bits, see alignment E=5.6e-65 PF13241: NAD_binding_7" amino acids 20 to 128 (109 residues), 72.2 bits, see alignment E=7e-24 PF10414: CysG_dimeriser" amino acids 163 to 220 (58 residues), 59.2 bits, see alignment 3.9e-20 TIGR01469: uroporphyrinogen-III C-methyltransferase" amino acids 228 to 462 (235 residues), 283.7 bits, see alignment E=1.4e-88 PF00590: TP_methylase" amino acids 229 to 439 (211 residues), 173 bits, see alignment E=1.2e-54

Best Hits

Swiss-Prot: 39% identical to CYSG_METFK: Siroheme synthase (cysG) from Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)

KEGG orthology group: K02302, uroporphyrin-III C-methyltransferase / precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 2.1.1.107 4.99.1.4] (inferred from 100% identity to atu:Atu1454)

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis or Coenzyme B12 biosynthesis or Dissimilatory nitrite reductase (EC 1.3.1.76, EC 2.1.1.107, EC 4.99.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.76, 2.1.1.107, 4.99.1.4

Use Curated BLAST to search for 1.3.1.76 or 2.1.1.107 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CZ78 at UniProt or InterPro

Protein Sequence (487 amino acids)

>Atu1454 siroheme synthase (Agrobacterium fabrum C58)
MHNEHDFSAAGDRLSTFPAFFRVEGRQVAVFGNGDEAFAKVRLLANTNAHIVAYADHPEA
DFARYISENSIDHRALAFASPLVDDATLVFAATGDEALDREIVSVARTKKIPANAVDQPE
FCDFFTPALVARAPVAIAIGTEGAGPVLAQMIRARIDQMLSPSLGKLARLAVQYRDAAEE
NVPKGALRRNFWRRFFSGAVADAVALGDTSSARHAADRLLQSPERSEGRVWLVGAGPGAE
DLLTLRAQRVLMEADVIVYDALVPQAIVDMGRRDAERLSVGKRKGCHSKSQNDINRLLVR
LGLEGKRVVRLKSGDPLVYGRAGEEMAALREAGVAYEIVPGITSAFAAAADFELPLTLRG
VASSLIFTTGHDLMGDVLPDWARLAVSGATIAVYMGRSVAASVAKRLIDAGLGVETTVAV
IENASRADRRLLHGTLNDLPDLEYRDELIGPVMVIIGDAVAGANFDRSEALALAAKPASL
KREYVNG