Protein Info for Atu1446 in Agrobacterium fabrum C58

Annotation: two component response regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 TIGR01818: nitrogen regulation protein NR(I)" amino acids 5 to 472 (468 residues), 698.5 bits, see alignment E=2e-214 PF00072: Response_reg" amino acids 5 to 114 (110 residues), 89 bits, see alignment E=7.4e-29 PF00158: Sigma54_activat" amino acids 138 to 304 (167 residues), 241.6 bits, see alignment E=1.1e-75 PF14532: Sigma54_activ_2" amino acids 139 to 309 (171 residues), 91.2 bits, see alignment E=2.2e-29 PF07724: AAA_2" amino acids 161 to 285 (125 residues), 30 bits, see alignment E=1.5e-10 PF07728: AAA_5" amino acids 162 to 280 (119 residues), 31.8 bits, see alignment E=4.1e-11 PF02954: HTH_8" amino acids 432 to 471 (40 residues), 50.6 bits, see alignment 3.7e-17

Best Hits

Swiss-Prot: 92% identical to NTRC_RHIME: DNA-binding transcriptional regulator NtrC (ntrC) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K07712, two-component system, NtrC family, nitrogen regulation response regulator GlnG (inferred from 100% identity to agr:AGROH133_05949)

Predicted SEED Role

"Nitrogen regulation protein NtrC"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CZ84 at UniProt or InterPro

Protein Sequence (483 amino acids)

>Atu1446 two component response regulator (Agrobacterium fabrum C58)
MTATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWVSAGEGDLVVTDVVMPDEN
AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIAIIGRALSE
PKRKPAKLDDDMQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALH
DYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQNRSTGRFEQAEGGTLFLDEIG
DMPMDAQTRLLRVLQQGEYTTVGGRTPIRTDVRIVAATNKDLKQSINQGLFREDLYYRLN
VVPLRLPPLRDRAEDIPDLVRHFIQTGEKEGLEGKRFETEALEVMKAYAWPGNVRELENL
IRRLMALYPQEVITREIIEQELQSDVPDSPLDKMAVRTGSLTISQAVEENMRDYFASFGD
GLPPPGLYDRVLRELEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYRSS
RPS