Protein Info for Atu1408 in Agrobacterium fabrum C58

Annotation: sorbitol dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 347 PF08240: ADH_N" amino acids 25 to 138 (114 residues), 111.7 bits, see alignment E=3.1e-36 PF16912: Glu_dehyd_C" amino acids 160 to 335 (176 residues), 43.5 bits, see alignment E=5.4e-15 PF00107: ADH_zinc_N" amino acids 179 to 303 (125 residues), 79.5 bits, see alignment E=4.4e-26 PF13602: ADH_zinc_N_2" amino acids 212 to 329 (118 residues), 33.2 bits, see alignment E=1.9e-11

Best Hits

Swiss-Prot: 36% identical to DHSO_BACSU: Sorbitol dehydrogenase (gutB) from Bacillus subtilis (strain 168)

KEGG orthology group: K00098, L-idonate 5-dehydrogenase [EC: 1.1.1.264] (inferred from 100% identity to atu:Atu1408)

MetaCyc: 36% identical to glucitol dehydrogenase monomer (Bacillus subtilis subtilis 168)
D-xylulose reductase. [EC: 1.1.1.9]; L-iditol 2-dehydrogenase. [EC: 1.1.1.9, 1.1.1.14]; 1.1.1.14 [EC: 1.1.1.9, 1.1.1.14]

Predicted SEED Role

"L-idonate 5-dehydrogenase (EC 1.1.1.264)" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.1.264)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.14, 1.1.1.264, 1.1.1.9

Use Curated BLAST to search for 1.1.1.14 or 1.1.1.264 or 1.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CZB5 at UniProt or InterPro

Protein Sequence (347 amino acids)

>Atu1408 sorbitol dehydrogenase (Agrobacterium fabrum C58)
MRGVVIHAAKDLRVEDVAGQPLAADEVRVAVAVGGICGSDLHYYNHGGFGTVRVREPMAL
GHEFAGTVVEVGSSVSHLVPGMRVAVNPSLPCGTCRYCAQGRQNQCLDMRFMGSAMRSPH
VQGGFREVVTVHSTQPVQIADGLSMGEAAMAEPLAVCLHAARQAGSLLGKTVLITGAGPI
GMLSLLVARLAGAAHIVVTDVADAPLDLARRIGADEAVNILRDADMLEKYRFEKGVFDVL
FEASGNQAALLPALDLLRPGGIIVQLGLGGDFTIPMNLIVAKELQLRGTFRFHEEFAQAV
NMMGRGLIDVKPLISATLPFDQAREAFDLAGDRAKSMKVQLAFSGAA