Protein Info for Atu1276 in Agrobacterium fabrum C58

Annotation: NADH ubiquinone oxidoreductase chain G

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 693 PF13510: Fer2_4" amino acids 3 to 69 (67 residues), 45.2 bits, see alignment E=2.9e-15 TIGR01973: NADH dehydrogenase (quinone), G subunit" amino acids 4 to 601 (598 residues), 733.2 bits, see alignment E=1.5e-224 PF00111: Fer2" amino acids 6 to 56 (51 residues), 30 bits, see alignment 1.4e-10 PF10588: NADH-G_4Fe-4S_3" amino acids 92 to 130 (39 residues), 62.7 bits, see alignment 6.3e-21 PF22117: Fer4_Nqo3" amino acids 145 to 211 (67 residues), 113.6 bits, see alignment E=1.4e-36 PF22151: Fer4_NDSU1" amino acids 225 to 274 (50 residues), 96.8 bits, see alignment 1.4e-31 PF00384: Molybdopterin" amino acids 280 to 603 (324 residues), 347.3 bits, see alignment E=3.5e-107 PF09326: NADH_dhqG_C" amino acids 637 to 680 (44 residues), 60.6 bits, see alignment 6.3e-20

Best Hits

Swiss-Prot: 59% identical to NQO3_PARDE: NADH-quinone oxidoreductase chain 3 (nqo3) from Paracoccus denitrificans

KEGG orthology group: K00336, NADH dehydrogenase I subunit G [EC: 1.6.5.3] (inferred from 100% identity to atu:Atu1276)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CZL6 at UniProt or InterPro

Protein Sequence (693 amino acids)

>Atu1276 NADH ubiquinone oxidoreductase chain G (Agrobacterium fabrum C58)
MAKLKVDGKEIEVPDHFTLLQACEEAGAEVPRFCFHERLSVAGNCRMCLIEVKGGPPKPA
ASCAMGVRDVRGGPNGELPEVFTNTPMVKKAREGVMEFLLINHPLDCPICDQGGECDLQD
QAMAFGIAGSRYAENKRAVEDKYIGPLVKTVMNRCIHCTRCVRFTTEVAGIAELGLIGRG
EDAEITTYLEQAMTSELQGNVVDLCPVGALTSKPFAFTARPWELNKTETIDVMDALGSAI
RVDTRGREVMRVMPRVNEAINEEWISDKSRFIWDGLKTQRLDRPYVRKDGRLQPATWGEA
FGAIKQAVAATSGSKIGAIAGDLSSVEEMFALKSLLASLGSANVDCRQDGAALDPSLGRA
SYIFNSTIEGIEHADALLIVGSNPRFEAAVLNARIRKRWRRGGFPIGVIGEAGELRYNYE
YLGSGAETLSDLANGSHSFIDKLKSAKNPLIIIGQGALARADGAAVLAAAAKLAVSVGAV
TEEWNGFSVLHTAAARVGGLDIGFVPGEGAVAAAEMVTSMDVLFLLGADEIDLSNKGAKF
TVYIGSHGDQGAMNADVILPGAAYTEKSGIWVNTEGRVQVGNRAGFAPGEAREDWAILRA
LSDVLGKKLPFDSLSALRGQLYVAHPHLAETDEIVAGKATDIEALAGKTGSLTKSAFASP
VKDFYLTNPIARASAVMAECSALARNNFQAAAE