Protein Info for Atu1261 in Agrobacterium fabrum C58

Annotation: ATP-dependent protease LA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 805 PF02190: LON_substr_bdg" amino acids 12 to 203 (192 residues), 188.1 bits, see alignment E=6.4e-59 TIGR00763: endopeptidase La" amino acids 14 to 772 (759 residues), 1003.1 bits, see alignment E=5.7e-306 PF08298: AAA_PrkA" amino acids 323 to 374 (52 residues), 28.5 bits, see alignment 3e-10 PF07728: AAA_5" amino acids 354 to 478 (125 residues), 35.6 bits, see alignment E=3.1e-12 PF00004: AAA" amino acids 354 to 491 (138 residues), 81.9 bits, see alignment E=2.1e-26 PF22667: Lon_lid" amino acids 514 to 566 (53 residues), 67.9 bits, see alignment 1.7e-22 PF05362: Lon_C" amino acids 571 to 773 (203 residues), 329.4 bits, see alignment E=2.8e-102 PF13541: ChlI" amino acids 619 to 741 (123 residues), 34.4 bits, see alignment E=6.6e-12

Best Hits

Swiss-Prot: 88% identical to LON_RHIME: Lon protease (lon) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 100% identity to atu:Atu1261)

MetaCyc: 64% identical to Lon protease (Escherichia coli K-12 substr. MG1655)
Endopeptidase La. [EC: 3.4.21.53]

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CJB5 at UniProt or InterPro

Protein Sequence (805 amino acids)

>Atu1261 ATP-dependent protease LA (Agrobacterium fabrum C58)
MTNITSAASGGTYPVLPLRDIVVFPHMIVPLFVGREKSIRALEEVMGSDKQIMLVTQINA
SDDDPAPEAIHKVGTVANVLQLLKLPDGTVKVLVEGKGRAQIDSYTGREDFYEASATPLQ
EPAEDPVEIEALSRSVVSEFESYVKLNKKISPEVVGAAGQIDDYSKLADTVASHLSIKIT
EKQEMLETVSVKQRLEKALGFMEGEISVLQVEKRIRSRVKRQMEKTQREYYLNEQMKAIQ
KELGDGEDGRDEMAELEERIAKTKLSKEAKEKADAEMKKLRQMSPMSAEATVVRNYLDWL
LGLPWGKKSKIKTDLNAAETILDQDHFGLDKVKERIVEYLAVQARATKIRGPILCLVGPP
GVGKTSLAKSIAKATGREYVRMALGGVRDEAEIRGHRRTYIGSMPGKIVQSMKKAKKANP
LFLLDEIDKMGMDFRGDPSSALLEVLDPEQNSTFMDHYLEVEYDLSDVMFVTTANTLNIP
GPLMDRMEVIRIAGYTEDEKREIAKRHLLPKAIKEHALRPEEFSVSDDALMAISQQYTRE
AGVRNFERELMKLARKAVTEIIKGKSTSVAVTAANIEDYLGIPRFRHGEAEREDQVGVVT
GLAWTEVGGELLTIEGVMMPGKGRMTVTGNLKEVMKESISAAASYVRSRAVDFGIEPPRF
DKSDIHVHVPEGATPKDGPSAGVAMATAIVSIMTGIPVSKDVAMTGEITLRGRVLPIGGL
KEKLLAALRGGIKKVLIPEENAKDLAEIPDNVKNEMEIIPVSRMGEVIKHALIRQPEPIE
WDGSIETPVIATVEGVDDGNQTIAH