Protein Info for Atu1030 in Agrobacterium fabrum C58

Annotation: GTP pyrophosphohydrolase/synthetase, RelA/SpoT family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 744 TIGR00691: RelA/SpoT family protein" amino acids 26 to 738 (713 residues), 644.8 bits, see alignment E=8.4e-198 PF13328: HD_4" amino acids 26 to 175 (150 residues), 165.8 bits, see alignment E=2e-52 PF01966: HD" amino acids 45 to 144 (100 residues), 37.1 bits, see alignment E=1e-12 PF04607: RelA_SpoT" amino acids 236 to 346 (111 residues), 138.6 bits, see alignment E=3e-44 PF02824: TGS" amino acids 393 to 450 (58 residues), 72.4 bits, see alignment 7.8e-24 PF19296: RelA_AH_RIS" amino acids 462 to 652 (191 residues), 262 bits, see alignment E=1.1e-81 PF13291: ACT_4" amino acids 664 to 741 (78 residues), 50.6 bits, see alignment E=7.3e-17

Best Hits

Swiss-Prot: 70% identical to RSH_BRUO2: GTP pyrophosphokinase rsh (rsh) from Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)

KEGG orthology group: K01139, guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [EC: 3.1.7.2] (inferred from 100% identity to atu:Atu1030)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7D045 at UniProt or InterPro

Protein Sequence (744 amino acids)

>Atu1030 GTP pyrophosphohydrolase/synthetase, RelA/SpoT family (Agrobacterium fabrum C58)
MMRQYELVERVQKYKPDANEALLNKAYVYAMQKHGQQKRANGDPYISHPLEVAAILTEMH
LDESTIAVALLHDTIEDTTATRAEIDELFGEDIGRLVEGLTKLKKLDLVTRKAKQAENLR
KLLLAISDDVRVLLVKLADRLHNMRTMEYMPADKRSRISEETMEIYAPLAGRMGMQDMRD
ELEDLSFRYLNPEAYETVTNRLLELETRNEGLIKKIEDELRELLVANGLLGTHVKGRQKK
PYSVFRKMQSKSLSFEQLSDVYGFRILVDDIPGCYRALGIVHTRWRVVPGRFKDYISTPK
QNDYRSIHTTIVGPSRQRIELQIRTKRMHEIAEFGIAAHALYKDGENGEGDLLSKESNAY
SWLRHTIESLAEGDSPEEFLEHTKLELFQDQVFCFTPKGKLIALPRGATPIDFAYAVHTN
IGDTTVGAKINGRIMPLVTRLNNGDEVEIIRSGVQVPPAAWEEVVVTGKARSAIRRATRM
AIRKQYSGLGYRILERTFERAGKAFSREALKPVLHRLAQKDVEDAIAAVGRGEVSSLDVL
RAVFPDYQDERVTVKMTGDDGWFNMRSASGMVFKIPGKSRSVLEDDGAAEMLDGPDPLPI
RGLSGNVDVHFSAAGAVPGDRIVGIMEKGKGITIYPIQAPALQRFDDEPERWIDVRWDLD
EANKSRFMARVMINALNEPGTLASVAQSIATLDVNIRGLNMVRIGTDFSELALDVEVWDL
RQLNQLLSQLKDLDCVSTVARAFD