Protein Info for Atu0981 in Agrobacterium fabrum C58

Annotation: glutamate-ammonia-ligase adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 988 PF03710: GlnE" amino acids 59 to 306 (248 residues), 167.6 bits, see alignment E=3.4e-53 amino acids 581 to 821 (241 residues), 214.7 bits, see alignment E=1.5e-67 PF08335: GlnD_UR_UTase" amino acids 328 to 468 (141 residues), 100 bits, see alignment E=1.2e-32

Best Hits

Swiss-Prot: 100% identical to GLNE_AGRFC: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 100% identity to atu:Atu0981)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UGQ4 at UniProt or InterPro

Protein Sequence (988 amino acids)

>Atu0981 glutamate-ammonia-ligase adenylyltransferase (Agrobacterium fabrum C58)
MTKRETVERRLSEVPPGVIRPYTQTELKSVFSTLKDIGKAEPAVAALLAGESPLKDFIAA
AFTLSPYLRDMAAADEGLLTLAISKPLEPLLTDLVRDARDCWKPAEDAVPVENEVMSRLR
IAKRRLSFVAALADLARIFTARDTTRWLSEMADASLSAAIDHLLLSAHESGKLKLKNLAA
PSEGSGLIVLGMGKLGARELNYSSDIDAVVFFEPSAGIIDDPYDATENFGRMMRRLVRIM
QERTADGYVFRTDLRLRPDPGSTPLAIPVEAALLYYEGRGQNWERAAYIKARPVAGDIKA
GENFLRELTPFIFRKYLDYAAIADIHSIKRQIHAHKGHGAIAVKGHNVKLGRGGIREIEF
FAQTQQLIAGGRMPPLRVRATEDALAALTEAKWIDAETRDSLTEAYWFLREVEHRIQMVR
DEQTHVLPDTEAELKRIAFMLGFEDTKAFSEKLEEVLRLVERRYSALFEQETKLSGEAGN
LVFTGQKDDPDTLKTLSTLGFQRPEDISRVIRTWHNGRYRATQSVEARERLTELTPDLLR
AFGESKRADEALLRFDNFLSGLPAGIQLFSLLGNNPALLSLLVTIMSSAPRLAEIIAARP
HVFDGMLDPALMSDVPTRDYLAHRMGNFLSNARHYEDILDRLRIFAAEQRFLIGVRLLTG
AIRGEVAARAFTHLADLVIEAALNAVLSEMEAAHGPYPGGRVAVMGMGKLGSFELTAGSD
VDLILLYDYDDTAQESTGAKPLDVVRYFTRVTQRLIAALSAPTAEGVLYEVDMRLRPSGN
KGPVATRISAFEKYQREEAWTWEHMALSRARLICGDASLMEDARSIIASILSQKRDVAKV
STDVLDMRSLIEQEKPPENNWDFKLINGGLIDLEFIAQYLALIGPVKGLGAHEPGRNTAE
ALQALAAPVMESQAFDDCMAAMGLYTEISQIVRLCIDGAFNPKEAPAGLIDLVCRAGDCP
DIPTLEGEVKRLSKAVRKAFVAVVKNGG