Protein Info for Atu0836 in Agrobacterium fabrum C58

Annotation: Glutathione-S-transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02798: GST_N
  • amino acids 2 to 85 (84 residues), 42.7 bits, E=1.7e-14
• PF13417: GST_N_3
  • amino acids 5 to 90 (86 residues), 40.1 bits, E=1.1e-13
• PF13409: GST_N_2
  • amino acids 12 to 85 (74 residues), 49.1 bits, E=2e-16
• PF00043: GST_C
  • amino acids 132 to 204 (73 residues), 52.4 bits, E=1.5e-17
• PF13410: GST_C_2
  • amino acids 137 to 200 (64 residues), 39.5 bits, E=1.5e-13
• PF14497: GST_C_3
  • amino acids 140 to 208 (69 residues), 35.4 bits, E=3.1e-12

Best Hits

KEGG orthology group: K00799, glutathione S-transferase [EC: 2.5.1.18] (inferred from 100% identity to atu:Atu0836)

Predicted SEED Role

"Maleylacetoacetate isomerase (EC 5.2.1.2) / Glutathione S-transferase" in subsystem Gentisare degradation or Homogentisate pathway of aromatic compound degradation or Salicylate and gentisate catabolism (EC 5.2.1.2)

MetaCyc Pathways

• L-tyrosine degradation I (3/5 steps found)
• 4-hydroxy-2-nonenal detoxification (1/4 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• pentachlorophenol degradation (4/10 steps found)
• gliotoxin biosynthesis (2/9 steps found)
• glutathione-mediated detoxification II (1/9 steps found)
• indole glucosinolate activation (intact plant cell) (3/12 steps found)
• camalexin biosynthesis (2/12 steps found)

KEGG Metabolic Maps

• Drug metabolism - cytochrome P450
• Glutathione metabolism
• Metabolism of xenobiotics by cytochrome P450
• Styrene degradation
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.18

Use Curated BLAST to search for 2.5.1.18 or 5.2.1.2

Sequence Analysis Tools

See A9CJU4 at UniProt or InterPro

Protein Sequence (219 amino acids)

>Atu0836 Glutathione-S-transferase (Agrobacterium fabrum C58)
MLTIYGVYRSRATRTLWLAAELGIEFKHVPVIQARRLADPLATDAPLNTLSPAFLAVNPM
GTIPCIEDDGMVLYESMAINLYLARKYGGPLAPVDLKEDAAMLQWSFFAATEIETNSLKI
SSAIAEGLAESDAGKAVIDVAARLLKRPLRVLEQHLATHDYLVGDRFTVADLNVAEIVRY
AQGHQPLFDAHPALKAWLARCQARTAFKSMWDSRTAEAA