Protein Info for Atu0644 in Agrobacterium fabrum C58

Annotation: glycyl-tRNA synthetase, beta chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 717 PF02092: tRNA_synt_2f" amino acids 4 to 574 (571 residues), 575.5 bits, see alignment E=1.4e-176 PF05746: DALR_1" amino acids 608 to 704 (97 residues), 34.1 bits, see alignment E=2.6e-12

Best Hits

Swiss-Prot: 100% identical to SYGB_AGRFC: Glycine--tRNA ligase beta subunit (glyS) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 100% identity to atu:Atu0644)

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CK39 at UniProt or InterPro

Protein Sequence (717 amino acids)

>Atu0644 glycyl-tRNA synthetase, beta chain (Agrobacterium fabrum C58)
MPDLLLELRSEEIPARMQRKAAGDLKKLVTDALVERGLTYEGAREYWTPRRLTLDIRGLN
ARSADVREEKKGPRTDANEKAIEGFLRGAGLNDISEAQVVSDPKKGDFYIAIINKPGRPA
EEIIAEVMPGIIRSFPWPKSMRSGPASMPKGSSYAGIEGKGSESLRWVRPLQSIVCLFGP
EHDETQVIPFVIDGIVAGNITYGHRFHAPGPITVRRFEDYVSNLEKAKVILDADRRKDII
LHDAKDLAFANGLELVEDEGLLEEVSGLVEWPQVLMGTFEEDYLQIPAEIIRLTIKTNQK
CFVTRNQGAEEGLSNRFILISNIEASDGGKEIIHGNGKVVRARLSDARHFWNRDQGDLPD
LETLKDSAAKFDLDLKKPLDQRMAKLDALNVTFHAKLGTQGERVARIRELAKALAPVVGA
DGALVDRAVVLAKADLRTEAVGEFPELQGLMGRKYAVLQGENESVAAAIEDHYKPQGPSD
RLPADKVAITVALADKLDTLVGFWAIDEKPTGSKDPFALRRAALGVVRILLEKNVRLPLL
SVARDSDLLSFFHDRLKVYLRDLGARYDLIDAVLTPESDDLLMIARRVEALTAFITGEDG
RNLLAGAKRATQLLAAEEKKGTVVADGVSEELLKLDAEKALYAAIKTASADAAKAVEGED
FRSAMQALSTLRAPVDKFFEDVLVNDEDAAIRANRLALLKAIREATGTVADFSKITG