Protein Info for Atu0607 in Agrobacterium fabrum C58

Annotation: isocitrate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00463: ICL
  • amino acids 14 to 243 (230 residues), 181.6 bits, E=2.4e-57
  • amino acids 242 to 424 (183 residues), 172.9 bits, E=1e-54
• TIGR01346: isocitrate lyase
  • amino acids 14 to 243 (230 residues), 314.6 bits, E=5.4e-98
• PF13714: PEP_mutase
  • amino acids 70 to 227 (158 residues), 54.8 bits, E=1.1e-18

Best Hits

Swiss-Prot: 70% identical to ACEA_BACHD: Isocitrate lyase (aceA) from Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

KEGG orthology group: K01637, isocitrate lyase [EC: 4.1.3.1] (inferred from 100% identity to atu:Atu0607)

MetaCyc: 67% identical to isocitrate lyase subunit (Mycobacterium tuberculosis H37Rv)
Isocitrate lyase. [EC: 4.1.3.1]

Predicted SEED Role

"Isocitrate lyase (EC 4.1.3.1)" in subsystem Serine-glyoxylate cycle (EC 4.1.3.1)

MetaCyc Pathways

• superpathway of glyoxylate bypass and TCA (12/12 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (12/14 steps found)
• glyoxylate cycle (6/6 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.3.1

Sequence Analysis Tools

See A9CK53 at UniProt or InterPro

Protein Sequence (429 amino acids)

>Atu0607 isocitrate lyase (Agrobacterium fabrum C58)
MTDFYKLVPGAPKGRFDGIERTYTAADVARLRGSVEIRHSLAEMGANRLWKLINEENFVN
ALGALSGNQAMQMVRAGLKAIYLSGWQVAADANTASAMYPDQSLYPANAAPELAKRINRT
LQRADQIETAEGKGLSVDTWFAPIVADAEAGFGGPLNAFEIMKAFIEAGAAGVHYEDQLA
SEKKCGHLGGKVLIPTAAHIRNLTAARLAADVMGVPTLVIARTDAEAAKLLTSDIDERDQ
PFVDYDAGRTVEGFYQVKNGLEPCIARAVAYAPYCDLIWCETSKPDLEQARRFAEGVHKV
HPGKKLAYNCSPSFNWKKNLDDATIAKFQRELGAMGYKFQFITLAGFHQLNFGMFELARG
YKDRQMAAYSELQEAEFAAEVNGYTATKHQREVGTGYFDAVSLAISGGTSSTTAMKESTE
HDQFRPAAE