Protein Info for Atu0598 in Agrobacterium fabrum C58

Annotation: phosphogluconate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 606 transmembrane" amino acids 134 to 152 (19 residues), see Phobius details amino acids 161 to 178 (18 residues), see Phobius details TIGR01196: phosphogluconate dehydratase" amino acids 4 to 604 (601 residues), 1053.9 bits, see alignment E=0 PF00920: ILVD_EDD" amino acids 68 to 601 (534 residues), 529 bits, see alignment E=6.6e-163

Best Hits

Swiss-Prot: 81% identical to EDD_RHIME: Phosphogluconate dehydratase (edd) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01690, phosphogluconate dehydratase [EC: 4.2.1.12] (inferred from 100% identity to atu:Atu0598)

MetaCyc: 62% identical to phosphogluconate dehydratase (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydratase. [EC: 4.2.1.12]

Predicted SEED Role

"Phosphogluconate dehydratase (EC 4.2.1.12)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CK56 at UniProt or InterPro

Protein Sequence (606 amino acids)

>Atu0598 phosphogluconate dehydratase (Agrobacterium fabrum C58)
MSADSRIQAITARIVERSKPYRETYLERLRLQVSKGVHRSVLSCGNLAHGFAVCSPADKD
ILAGDRVPNLGIITAYNDMLSAHQPYETFPAIIRDAAKEAGGIAQVAGAVPAMCDGVTQG
QPGMELSLFSRDAIAMAAGIGLSHNMFDAAVYLGVCDKIVPGLVIAALAFGHLPAVFVPA
GPMTSGLPNDEKSRIRQLYAEGKVGRAELLEAESKSYHGPGTCTFYGTANSNQMLMEIMG
FHMPGSSFINPGTPLRDALTREAANRALAITAQGNEFTPAGEMIDERSVVNGVVGLHATG
GSTNHTMHLIAMARAAGIILTWQDISDLSDIVPLLARVYPNGLADVNHFHAAGGMGFLIK
QLLKQGFVHDDVRTVFGQGLSAYTVDAMLDEKGAVTRQPSPEQSHDPKVLSSIETPFQST
GGLKMLTGNLGKSVIKISAVKPERHIIEAPAIVFHDQQELQDAFKDGKLNRDFIAVVRFQ
GPKANGMPELHRLTPPLGVLQDRGFKVALVTDGRMSGASGKVPAAIHVTPEASDCGPISL
IRDGDIIRLDAISGTLEVLVSAAELAKREPARADLSGNEWGMGRELFAPFRRNAGPADQG
ASVLFH