Protein Info for Atu0523 in Agrobacterium fabrum C58

Annotation: flagellar M-ring protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 566 transmembrane" amino acids 28 to 49 (22 residues), see Phobius details amino acids 445 to 464 (20 residues), see Phobius details TIGR00206: flagellar M-ring protein FliF" amino acids 29 to 430 (402 residues), 199.5 bits, see alignment E=5.9e-63 PF01514: YscJ_FliF" amino acids 50 to 223 (174 residues), 169.1 bits, see alignment E=9.6e-54 PF08345: YscJ_FliF_C" amino acids 255 to 420 (166 residues), 115.6 bits, see alignment E=2.7e-37

Best Hits

Swiss-Prot: 71% identical to FLIF_RHIME: Flagellar M-ring protein (fliF) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K02409, flagellar M-ring protein FliF (inferred from 100% identity to atu:Atu0523)

Predicted SEED Role

"Flagellar M-ring protein FliF" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7D1A2 at UniProt or InterPro

Protein Sequence (566 amino acids)

>Atu0523 flagellar M-ring protein (Agrobacterium fabrum C58)
MTVRNRMNLLNQIPQVLKNVAALGQTRLLMLGGVGVLSMAIILAAALYVNRPAYETLYVG
LEKSDLNKISIALAESGLDFQVGTDGSSLQVPVGLTSKARLLLAERGLPDSANAGYELFD
NVGSLGLTSFMQEVTRVRALEGEIGRSIQQIDGVSAARVHIVLPDVGNFRRGEQKPTASV
MIRASASAGRKASASIRHLVASAVPGLEVDDVTLLDSTGQLLASGDDVTNAAMNRSLTLA
QNVQQEITTNIDKALAPFLGIDNFRSSVTAQINTDSRQVQETVYDPESRVERSVRTVKED
QKSQETQPDTAATVEQNVPQAAPQGGGGGPQSSDQSAKKEEQTNYEINSKTVATVKNGYT
LEKISVAVVVNKGRIAKMVGEPADQAKIDAYLAEMQKIVTSAAGISSDRGDIVTLTAMDF
LETQLLDEAASGPGVMEVLSRNSAGIINSLAFVAVAFLVVWLGVRPLVRTVTANGSAAAQ
LSQESAGLELPDFSPGMEAGAGGLMEGFGADFGFDSTDDLLAGGDGEGTFNRRVREGPER
RLSRMVEISEERAAKILRKWAVEKAA