Protein Info for Atu0509 in Agrobacterium fabrum C58

Annotation: phaE protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 161 transmembrane" amino acids 9 to 44 (36 residues), see Phobius details amino acids 59 to 83 (25 residues), see Phobius details amino acids 103 to 122 (20 residues), see Phobius details PF01899: MNHE" amino acids 12 to 159 (148 residues), 135.8 bits, see alignment E=4.8e-44

Best Hits

Swiss-Prot: 42% identical to PHAE_RHIME: Probable K(+)/H(+) antiporter subunit E (phaE) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K05562, multicomponent K+:H+ antiporter subunit E (inferred from 100% identity to atu:Atu0509)

Predicted SEED Role

"Na(+) H(+) antiporter subunit E" in subsystem Sodium Hydrogen Antiporter

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7D1B4 at UniProt or InterPro

Protein Sequence (161 amino acids)

>Atu0509 phaE protein (Agrobacterium fabrum C58)
MSRLLPYPLLTVSLIFFWLTINSFSAGHLLLGTGVALIASWAMASLRPAKPRIRNWHRLV
QLILIVLYDIVRSNLSVAKIILFQRERDRKSGFLAVPLEIRDPMALAVLATILTSTPGSA
WLEYNSSQGTLLLHVLDDVDEAAWISLIKNRYEKLLMEIFE