Protein Info for Atu0503 in Agrobacterium fabrum C58

Annotation: enoyl-CoA hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 738 transmembrane" amino acids 333 to 350 (18 residues), see Phobius details PF00378: ECH_1" amino acids 14 to 215 (202 residues), 101.6 bits, see alignment E=1.2e-32 PF16113: ECH_2" amino acids 25 to 214 (190 residues), 79.6 bits, see alignment E=8.3e-26 PF02737: 3HCDH_N" amino acids 329 to 508 (180 residues), 200.6 bits, see alignment E=5.2e-63 PF03446: NAD_binding_2" amino acids 329 to 445 (117 residues), 22.3 bits, see alignment E=3.1e-08 PF00725: 3HCDH" amino acids 510 to 607 (98 residues), 71.6 bits, see alignment E=1.6e-23

Best Hits

KEGG orthology group: K01782, 3-hydroxyacyl-CoA dehydrogenase / enoyl-CoA hydratase / 3-hydroxybutyryl-CoA epimerase [EC: 1.1.1.35 4.2.1.17 5.1.2.3] (inferred from 100% identity to atu:Atu0503)

Predicted SEED Role

"Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Isobutyryl-CoA to Propionyl-CoA Module (EC 1.1.1.35, EC 4.2.1.17, EC 5.1.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.1.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CK81 at UniProt or InterPro

Protein Sequence (738 amino acids)

>Atu0503 enoyl-CoA hydratase (Agrobacterium fabrum C58)
MSTYTNFTIETDADGIALVTWDMPEKSMNVFTAEVLEELNAIVDATVADGAVKGVVFTSG
KSSFSGGADLSMLKSMFALYNDEKAKSPDAAAAKLFELVGRMTGLFRKIETNGKPWVSAI
NGTCMGGAFELSLACHGRVASSAKSLKIALPEVKVGIFPGAGGTQRVSRLTDAQSALQMM
TTGQSLTGARAKAMGLVHQVVEPDQLVATAKQMIKDGLKPVAPWDEKGFKAPGGGIWTPA
AAQLWPAAPAILRRESAGNYPAALAILKCVYEGLQLPFDTALKVEQRYFTEILRSKEAFG
MIRSLFISMQELNKGARRPAGQPKTEFKKVGVVGAGFMGAAVAYVTAAAGIPVTLVDRDQ
DAADKGKGHCEESVKAAIGKGRLTQEEGKALLDLVTPTSDYATLADADLVIEAVFEDRDV
KKAVIEAVEAVLPEGAIFASNTSTLPITGLAKNSKRPADFIGIHFFSPVEKMMLTEVILG
KETGDKALAVALDYVAKIRKTPIVVNDTRGFFVNRCVLRYMAESYDMLIEGVPAAMIENA
AKFAGMPVGPLALNDEVAIDLSYKILKATVADLGEKAVDPRHMELVKKLVEGDGRFGRKN
AKGFYDYPPKPAKKSLWPGLKDLYPQQKPEDVDMDVLKQRFLVTVALEAARTVEEGIVTD
PREADVGSILGFGFAPYTGGALSYIDGMGVKNFVALAEKLSEAYGPRFQPTPLLKDMAAK
GETFYGRFDPYASAAKAA