Protein Info for Atu0368 in Agrobacterium fabrum C58

Annotation: tRNA pseudouridine synthase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 266 TIGR00071: tRNA pseudouridine(38-40) synthase" amino acids 4 to 241 (238 residues), 193.7 bits, see alignment E=1.9e-61 PF01416: PseudoU_synth_1" amino acids 7 to 107 (101 residues), 51.5 bits, see alignment E=6.4e-18 amino acids 146 to 248 (103 residues), 100.9 bits, see alignment E=2.7e-33

Best Hits

Swiss-Prot: 100% identical to TRUA_AGRFC: tRNA pseudouridine synthase A (truA) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K06173, tRNA pseudouridine synthase A [EC: 5.4.99.12] (inferred from 100% identity to atu:Atu0368)

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UIC9 at UniProt or InterPro

Protein Sequence (266 amino acids)

>Atu0368 tRNA pseudouridine synthase I (Agrobacterium fabrum C58)
MPRFRMTVEYDGTPYYGWQRQENGPSVQGALEAAVRSLTGESVSIRGAGRTDSGVHAVGQ
VAHIDLSRDWEPYKLRNALNAHLAMAGEAVAVLDAAAVTEDFDARFSALRRHYLYRIICR
KSRLALEHKRAWWVSKDLDHERMHEAAQMLVGRHDFTTFRSVHCQANSPVRTLDRLDVTR
NGDLIEIRATAQSFLHNQIRSFAGTLKLAGEGGMTPDDVRAALEARDRKACGPVAPPDGL
YFMQVDYPDVIPPRRRPEIEMTEDAD