Protein Info for Atu0356 in Agrobacterium fabrum C58

Annotation: miaB protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 455 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 11 to 449 (439 residues), 488.7 bits, see alignment E=1.5e-150 TIGR01574: tRNA-i(6)A37 thiotransferase enzyme MiaB" amino acids 11 to 452 (442 residues), 494.8 bits, see alignment E=2.6e-152 PF00919: UPF0004" amino acids 11 to 114 (104 residues), 106.2 bits, see alignment E=1.1e-34 PF04055: Radical_SAM" amino acids 167 to 338 (172 residues), 101.1 bits, see alignment E=1.2e-32 PF01938: TRAM" amino acids 393 to 452 (60 residues), 36.8 bits, see alignment E=4.5e-13

Best Hits

Swiss-Prot: 100% identical to MIAB_AGRFC: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (miaB) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K06168, bifunctional enzyme involved in thiolation and methylation of tRNA (inferred from 100% identity to atu:Atu0356)

MetaCyc: 50% identical to isopentenyl-adenosine A37 tRNA methylthiolase (Escherichia coli K-12 substr. MG1655)
RXN0-5063 [EC: 2.8.4.3]

Predicted SEED Role

"tRNA-i(6)A37 methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase or tRNA processing

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7D1M2 at UniProt or InterPro

Protein Sequence (455 amino acids)

>Atu0356 miaB protein (Agrobacterium fabrum C58)
MIAREGSNSRKVFIKTYGCQMNVYDSVRMSDALAKDGYVQTEDMGEADLVLLNTCHIREK
AAEKVYSALGRLRDMKKSREEQGREFMIGVAGCVAQAEGEEILRRAPAVDVVIGPQTYHR
LPDALKRVRRGERVIETEYAVEDKFEHLPVAEKATLRSRGVTAFLTVQEGCDKFCTFCVV
PYTRGSEVSRPVRQIVDEAMKLVDAGVREITLLGQNVNAWQGEGPKGEKWGLAELLYRLA
EIPGLARLRYTTSHPRDMDDRLIGAHRDLRILMPYLHLPVQSGSDRILKAMNRRHTGEEY
IQLIEKIRSARPDIAMSGDFIVGFPGETDRDFEDTMAMVETVKYAQAFSFKYSTRPGTPG
ADLTDQVAEDVKAERLERLQALLLRQQKEFAESLVGKTMDVLLEKPGRMPEQLIGRSPWL
QSVNLDAKTLKIGDIVNVRITATGPNSLFAEVAGS