Protein Info for Atu0330 in Agrobacterium fabrum C58

Annotation: nitrogen regulatory IIA protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01419: PTS IIA-like nitrogen-regulatory protein PtsN
  • amino acids 3 to 146 (144 residues), 170.8 bits, E=1.7e-54
• PF00359: PTS_EIIA_2
  • amino acids 6 to 147 (142 residues), 120.7 bits, E=2.5e-39

Best Hits

Swiss-Prot: 67% identical to PTSN_BRADU: Nitrogen regulatory protein (ptsN) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K02806, PTS system, nitrogen regulatory IIA component [EC: 2.7.1.69] (inferred from 99% identity to agr:AGROH133_03493)

Predicted SEED Role

"PTS system nitrogen-specific IIA component, PtsN"

KEGG Metabolic Maps

• Aminosugars metabolism
• Ascorbate and aldarate metabolism
• Fructose and mannose metabolism
• Galactose metabolism
• Glycolysis / Gluconeogenesis
• Nucleotide sugars metabolism
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

See A9CKE0 at UniProt or InterPro

Protein Sequence (153 amino acids)

>Atu0330 nitrogen regulatory IIA protein (Agrobacterium fabrum C58)
MALADLLQQDAIIPALKVNSKKQLLQELAAKAARITGVSEREVFDVILQREKLGSTGVGH
GIAIPHGKLNSIHQITGVFARLETPVDFEALDDQPVDLVFLLLAPEGAGADHLKALSRIA
RALRDPELVTKLRATDSDTAIYAFLNQEQATAA