Protein Info for Atu0321 in Agrobacterium fabrum C58

Annotation: formamidopyrimidine-DNA glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 298 PF01149: Fapy_DNA_glyco" amino acids 1 to 133 (133 residues), 116.6 bits, see alignment E=1.5e-37 TIGR00577: DNA-formamidopyrimidine glycosylase" amino acids 1 to 297 (297 residues), 256.4 bits, see alignment E=1.6e-80 PF06831: H2TH" amino acids 150 to 245 (96 residues), 73.1 bits, see alignment E=2.3e-24 PF06827: zf-FPG_IleRS" amino acids 268 to 298 (31 residues), 29.7 bits, see alignment 7e-11

Best Hits

Swiss-Prot: 100% identical to FPG_AGRFC: Formamidopyrimidine-DNA glycosylase (mutM) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K10563, formamidopyrimidine-DNA glycosylase [EC: 3.2.2.23 4.2.99.18] (inferred from 100% identity to atu:Atu0321)

Predicted SEED Role

"Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23)" in subsystem DNA Repair Base Excision (EC 3.2.2.23)

Isozymes

Compare fitness of predicted isozymes for: 4.2.99.18

Use Curated BLAST to search for 3.2.2.23 or 4.2.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UIH4 at UniProt or InterPro

Protein Sequence (298 amino acids)

>Atu0321 formamidopyrimidine-DNA glycosylase (Agrobacterium fabrum C58)
MPELPEVETVRRGLAPAMEGARVRKLHLGRPDLRFPFPADFAGLIEGKTIISLGRRAKYL
LIELEDGLTIVSHLGMSGSFRIEAEKGEGGEAPGAFHYARSKDGKHDHVVFHLDKGEERV
CVIYNDPRRFGFMHLVERNKLDLYPAFAELGPEPTGNALSADYLASRFEGKSQPLKSTLL
DQKIIAGLGNIYVCEALWRAHLSPLRAAGTLVTTRGKPKAQLIDLTEKIRDVIADAIAAG
GSSLRDHIQTDGTLGYFQHSFSVYDQEGQPCRTPGCGGTVERVVQAGRSTFYCAACQK