Protein Info for Atu0296 in Agrobacterium fabrum C58

Annotation: NADH-ubiquinone oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 326 transmembrane" amino acids 6 to 24 (19 residues), see Phobius details amino acids 247 to 263 (17 residues), see Phobius details PF05368: NmrA" amino acids 10 to 102 (93 residues), 32.6 bits, see alignment E=1.7e-11 PF04321: RmlD_sub_bind" amino acids 10 to 236 (227 residues), 46.8 bits, see alignment E=6.5e-16 PF01370: Epimerase" amino acids 10 to 218 (209 residues), 60.9 bits, see alignment E=4e-20 PF01073: 3Beta_HSD" amino acids 12 to 115 (104 residues), 29.3 bits, see alignment E=1.3e-10 amino acids 150 to 200 (51 residues), 22.5 bits, see alignment 1.6e-08 PF13460: NAD_binding_10" amino acids 14 to 133 (120 residues), 42.3 bits, see alignment E=2.3e-14

Best Hits

KEGG orthology group: K00329, NADH dehydrogenase [EC: 1.6.5.3] K00356, NADH dehydrogenase [EC: 1.6.99.3] (inferred from 100% identity to atu:Atu0296)

Predicted SEED Role

"NADH-UBIQUINONE OXIDOREDUCTASE 39 KD SUBUNIT (EC 1.6.5.3)" (EC 1.6.5.3)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3, 1.6.99.3

Use Curated BLAST to search for 1.6.5.3 or 1.6.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7D1R4 at UniProt or InterPro

Protein Sequence (326 amino acids)

>Atu0296 NADH-ubiquinone oxidoreductase (Agrobacterium fabrum C58)
MTLANLPPLVTVFGGSGFVGRHVVRMLAKRGYRIRVAVRRPDLAGFLQPLGNVGQISFAQ
ANLRYRDSIIKAVEDADHVVNCVGILAESGRNTFDAVQEFGAKAIAEAARDTGATLTHIS
AIGADANSQTGYGRTKGRAEAAIHSVLPGAVILRPSIIFGPEDDFFNKFAKMARNLPFLP
LIGGGKTKFQPVYVEDVAEAVARSVDGKLKPGAIYELGGPDVMTFRDCLEAVLAATYRER
SFVNLPFGVASMIGKLASLVPLITPPLTPDQVTMLKKDNVVSAEAEKKGLTLEGIGITPV
RVASVLPSYMVQYRQHGQFSNAGKAA