Protein Info for Atu0221 in Agrobacterium fabrum C58

Annotation: component of type IV pilus

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 527 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details PF13629: T2SS-T3SS_pil_N" amino acids 65 to 132 (68 residues), 67.1 bits, see alignment E=1.4e-22 PF04972: BON" amino acids 138 to 196 (59 residues), 32.1 bits, see alignment 1.7e-11 PF00263: Secretin" amino acids 297 to 475 (179 residues), 124.4 bits, see alignment E=5.6e-40

Best Hits

KEGG orthology group: K02280, pilus assembly protein CpaC (inferred from 100% identity to atu:Atu0221)

Predicted SEED Role

"Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly" in subsystem Widespread colonization island

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CKJ5 at UniProt or InterPro

Protein Sequence (527 amino acids)

>Atu0221 component of type IV pilus (Agrobacterium fabrum C58)
MTGGLMTKRFKHHLRSSMAGGLAFCLTFSGVPGRHSPDFLRTSEAEAQSASIVRITESGT
GIRKRLKLGLNKALVIDLPEDAHDILVADPSLADAVTRTSRRIYLFGKMVGQTNIFIFGD
GGREIVSLDLEVERDISGLEANIRRFIPESDIKVEIVSDNIVLTGSVRTPQDSARAVQLA
DAFLKGGEATTRNISLTGGNNGGDAAIFAERRQVSQIVNMLTIEGEDQVTLKVTVAEVSR
QVLKQLGFNGSISSSTSGNGFEFANPSNLGNAIDGASRIASGAIGSGSLRFASYLNAMEQ
AGVMRTLAEPSLTAISGEQAKFYVGGDFRLAAEQEVDIDKETGRPTITRTTNTVDYGITL
NFKPVVLSPGRISLKIETNVSEPTYEGNVVTGNGGRTIPGSTYMSVRKRETSTTVELPSG
GSIVIAGLVQDNVRQAMSGLPGMSKIPIFGTLFRSKEFLRNETELVIIATPYLVRPVARN
QIARPDDNFNPENDAAMYFMNRVNKVYGRKDQVQAAPYQGSVGFIYK