Protein Info for Atu0146 in Agrobacterium fabrum C58

Annotation: sulfite reductase (NADPH) flavoprotein alpha-component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00258: Flavodoxin_1
  • amino acids 54 to 184 (131 residues), 108.2 bits, E=6.2e-35
• PF00667: FAD_binding_1
  • amino acids 221 to 414 (194 residues), 85.7 bits, E=5.5e-28
• PF00175: NAD_binding_1
  • amino acids 447 to 553 (107 residues), 53.1 bits, E=6.9e-18

Best Hits

KEGG orthology group: K00380, sulfite reductase (NADPH) flavoprotein alpha-component [EC: 1.8.1.2] (inferred from 100% identity to atu:Atu0146)

Predicted SEED Role

"Sulfite reductase [NADPH] flavoprotein alpha-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.8.1.2

Sequence Analysis Tools

See A9CKN2 at UniProt or InterPro

Protein Sequence (589 amino acids)

>Atu0146 sulfite reductase (NADPH) flavoprotein alpha-component (Agrobacterium fabrum C58)
MMKIPYIPQDAPFNGDQRAWLAGFLAGLHSRTAIGLETSVAAAAPKSAASVLNILVGTQT
GNAEALAMDIAAAARTQGMQPVVTALDDVSMDSLGSMKRVIVVTSTYGEGEMPDNAQLFW
EALAADSAPRLDEMNFAVLALGDTGYDGFCQAGKLIDTRFEQLGGRRMKARIDCDIDFED
AAAAWIGEALPLALAVDGGKEAAVTMPDIAAQPTRAKSGWSRKNPYPATVGVNRRLSGSG
SSKEIRHYEFDLRESGLDYEAGDALGVVPLNNPKLAGALLGHFGAVGDSQVAGLDRPVGD
ALLNIYEISTPSRELVAEIGKRAGHEELSHILANGDKEQLAAFLWSKDILDLLNLGGKPL
FELAEFVTFLKPLQHRAYSISSSPLLAKDSVHLTIASVRYRAEGRERGGVCSTYLADRVE
AGQSAGIFVSPNKAFRVPQDDNAPVIMVGPGTGIAPFRAFLQERQARGARGKNWLFFGDQ
HRQSDFIYENELGDMSRDGVLTRLDLAFSRDQAEKIYVQTRMRQNGKALYQWLEEGGYFY
VCGDATRMAKDVDDALHSIISDEAGISAEAASDYVNRLKREKRYLRDVY