Protein Info for Atu0145 in Agrobacterium fabrum C58
Annotation: glutamate synthase large subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00265, glutamate synthase (NADPH/NADH) large chain [EC: 1.4.1.13 1.4.1.14] (inferred from 100% identity to atu:Atu0145)Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- ammonia assimilation cycle I (2/2 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-glutamate biosynthesis IV (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- superpathway of ammonia assimilation (plants) (2/3 steps found)
- L-glutamate and L-glutamine biosynthesis (4/7 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14
Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q7D240 at UniProt or InterPro
Protein Sequence (1782 amino acids)
>Atu0145 glutamate synthase large subunit (Agrobacterium fabrum C58) MSAEGVGDGAGVSLDLSLSFFRELTGDAALPAGRFGVGNFFLPQNPAFHGEAVGIIGNAL KEQGFSVLLVRDVPVNDTAIRPAAIPYQLPIRQWVFSAPVECATLAEFDWRIHKALLAIE ALAYTVPDLAGFYPLSLSARTQVLKGRLNSQEVMPYFCDLTDPRHKVHTMYFHTRFSTNT DPHPSMAQPFRLMAHNGELNTDKKNRLSEAAVALAKNASIVRPKGQSDSCRLDQTLQARV MEDGLDLVTAVVSMMPPAWENDDTLSPGVKSMLEYFSLYEEKNDGPAALIFGDGTIIGAR LDRLGLRPLRTVETDDYLCVMSEAGQIAFPAESVIRRGRIEAGGMLYYDHTERRAFSTVE ALELLASRRDYSSLLSDARVMLGDLPEVPADKQGSPLRYNGDLERHQRYVAYSHNQESFK FLMDPMLASGAEKISAMGYGNAINALSDQEGGVAKYFSQRFAQVTNPPLDSIREADGMTL RVALGAKPNSGAKKARQIVVRSPILTHLDMLRIREQPETPVRRFEMLYTPVFDAEDANEA ALRQAIDGLCGEVVAFAAEEGGIAVVTDRHVSAGRAALPMIMVVSAINQRLIEEGLRLRI SLIVESGQFSSSHHIAAGLGFGASAVYPLGVQFRAEEKFGADADKAFKRFAKAAEKSLMK TMGKVGLCTAESYIGGEFFEPNFLDTSDPVLKRYFPNVKTPVGGVTFAVIAQAVADWHRK ALSVKGENDIPLLGLFKERAEGAGHSYGTTAVRGFVDMTEEKIGFDKGTENEEALRLLPL NRLEDAFGLNDAAYYHNSFDRLTPDAIDAFEVTPGYRAFSRMMAEERARRPAALRDVLEL PADVTFAGSAEEFRREMGRFCRHGNNSFMVRGLRCDEAADGSFRLQLIGPHGHELARLAA LGQSLLDRFDDDIAGHWLEGGALMVQAKGEAFAYMSLIRTAPNSIPLETVQKASEITKTL ASGAMSHGALVAAAHEAVAHGTNMVGGMSNSGEGGEHISRYGTIRASRIKQFASGRFGVW AGYLADPMLEEIEIKIGQGAKPGEGGQLPSPKVTVEIAAARGGTPGVELVSPPPHHDTYS IEDLAQLIHDAKAARVRVIVKLVSSEGIGTIAVGVAKAGADVINVAGNTGGTGAAAVTSL KYTGRAAEIGIAEVHQALCATGLRAKVLLRCSGAHQTASDVVKSALLGGDSFEFGTTALM MLKCVMAKNCNIKCPAGLTTNQEAFNGDPRALAQYLMNIAHETREILAGLGLRSLREARG RSDLLHLLDHPASVGQLDLRAMLAVVEEVKIDNPVYMEKDFALDDAFIELVRSALVDSRR ENIELGGNRFLNNCNKSVGGQLAVDIERMLNHELSDEQLDALPAVKRDSRGRRFLEAGSV RIDTSGSAGQSFGAFCNDGMIMVHTGTCNDGVGKSACGGTIVVRSPGGGSKETGGNVLVG NFALFGATGGRTFVEGQAGDRFAVRNSGATAVVEGVGDFACEYMTNGAVLNLGSFGKGFG NGMSGGFVYQYDPYGDLPKKVSHDSILLGSITGEDEQAAIHNQAVHQLLTLHVAETGSAK AAWLLENWESEQHNFAYGMPRALLLYQDSDEILKAKPRKELLEELASALAGYQLRKFKLA YRDRKAVLNGTVPGYGETDTEDMYALINNYTVLSMAQAIALQRLPMASGPSDPAIEKAVR NLILTEDYALMQRLLKYAREALSDHSDQQLAVMIAAKRLDDYKQALARRNIWSVDSPGTY GWIIQQNRKNIEKIGRLPGFEELFAARAIPDLATASSHSRAA